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Related Experiment Video

Updated: Mar 17, 2026

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CATO: The Clone Alignment Tool.

Peter V Henstock1, Peter LaPan2

  • 1Pfizer R&D Business Technologies, 1 Burtt Road, Andover, MA, United States of America.

Plos One
|July 27, 2016
PubMed
Summary

High-throughput cloning generates many sequences requiring analysis. CATO, the Clone Alignment Tool, integrates alignment, editing, and selection into one software, streamlining cloning workflows.

Area of Science:

  • Molecular Biology
  • Bioinformatics
  • Genomics

Background:

  • High-throughput cloning generates vast sequence data.
  • Existing workflows require multiple software tools for sequence analysis.
  • Efficient management of clone sequences is crucial for research.

Purpose of the Study:

  • To develop a unified software solution for analyzing high-throughput cloning sequences.
  • To streamline the process of sequence alignment, editing, and selection.
  • To facilitate the integration of clone sequence analysis into existing bioinformatics pipelines.

Main Methods:

  • Developed CATO (Clone Alignment Tool), a single software application.
  • Implemented sequence alignment algorithms comparing candidate sequences to reference sequences.

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  • Integrated multi-level visualization tools for sequence analysis.
  • Ensured compatibility with standard data formats for pipeline integration.

    • Main Results:

    • CATO enables alignment, evaluation, editing, and selection of clone sequences.
    • Provides three levels of visualization: summary, focused alignment, and pair-wise alignment.
    • Allows users to define matching criteria and edit reference sequences.
    • Outputs results to a summary file for workflow integration.

    Conclusions:

    • CATO simplifies and accelerates the analysis of high-throughput cloning experiments.
    • Offers a comprehensive solution for managing and validating clone sequences.
    • Enhances the efficiency of molecular cloning and genomic research workflows.