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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Updated: Mar 17, 2026

Qualitative and Quantitative Analysis of the Immune Synapse in the Human System Using Imaging Flow Cytometry

Qualitative and Quantitative Analysis of the Immune Synapse in the Human System Using Imaging Flow Cytometry

Published on: January 7, 2019

Quantitative Functional Morphology by Imaging Flow Cytometry.

Ivan A Vorobjev^1,2, Natasha S Barteneva^3,4

¹Department of Cell Biology and Histology, M.V. Lomonosov Moscow State University, Moscow, 119992, Russia. vorob@libro.genebee.msu.ru.

Methods in Molecular Biology (Clifton, N.J.)

|July 28, 2016

Summary

Imaging flow cytometry (IFC) offers a hybrid approach for cell analysis, combining morphometric and fluorescent data. This technology presents advantages and limitations compared to conventional methods, with future directions explored.

Keywords:

Autofluorescence Automated image analysis Flow cytometry Imaging flow cytometry Quantitative morphology Spectral compensation

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Last Updated: Mar 17, 2026

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Area of Science:

Cellular imaging and analysis
Quantitative biology
Biophysical techniques

Background:

Conventional flow cytometry and fluorescent microscopy have limitations in detailed cellular analysis.
Imaging flow cytometry (IFC) integrates principles of both techniques for enhanced cellular characterization.

Observation:

The Imagestream instrumentation utilizes a hybrid approach for cell and particle analysis.
This method combines morphometric measurements with the quantitation of multiparametric fluorescent intensities' distribution.

Findings:

IFC provides a detailed view of cellular morphology and fluorescence distribution simultaneously.
The study outlines the specific advantages and limitations of IFC technology.

Implications:

IFC offers a powerful alternative for complex biological sample analysis.
Understanding IFC's capabilities and constraints informs its application in research and diagnostics.