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Updated: Mar 17, 2026

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy ATOM

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy ATOM

Published on: June 28, 2017

Ultrafast Microfluidic Cellular Imaging by Optical Time-Stretch.

Andy K S Lau¹, Terence T W Wong^1,2, Ho Cheung Shum³

¹Department of Electrical and Electronic Engineering, Faculty of Engineering, The University of Hong Kong, Pokfulam Road, Pokfulam, Hong Kong.

Methods in Molecular Biology (Clifton, N.J.)

|July 28, 2016

Summary

This summary is machine-generated.

Optical time-stretch microscopy enables ultra-fast, label-free single-cell imaging and analysis. This high-throughput technology overcomes limitations of current flow cytometry, offering detailed cellular insights for big-data applications.

Keywords:

Imaging flow cytometry Microfluidic fabrication Optofluidics Quantitative phase imaging Time-stretch microscopy Ultrafast label-free imaging

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Use of Dual Optical Tweezers and Microfluidics for Single-Molecule Studies

Use of Dual Optical Tweezers and Microfluidics for Single-Molecule Studies

Published on: November 18, 2022

Area of Science:

Biomedical Engineering
Optical Physics
Cell Biology

Background:

High-content, high-throughput single-cell analysis is crucial for big-data applications in pathology and drug discovery.
Current flow cytometry advancements offer more measurable parameters but face a trade-off between content and throughput.
Imaging flow cytometry achieves higher content but at significantly reduced throughput (~1000 cells/s).

Purpose of the Study:

Introduce optical time-stretch microscopy as a novel platform for ultra-fast, high-content single-cell analysis.
Address the unmet need for efficient measurement of multiple cellular parameters in large populations.
Enable label-free imaging and quantitative analysis of cellular properties at high throughput.

Main Methods:

Developed an optical time-stretch microscopy platform for label-free single-cell imaging.
Utilized ultrafast microfluidics enabling flow speeds up to 10 m/s.
Achieved ultra-fast imaging line-scan rates in the tens of MHz.
Integrated conventional fluorescence measurements.

Main Results:

Demonstrated ultrahigh-speed, high-contrast label-free imaging of single cells.
Enabled quantitative evaluation of cellular parameters (volume, mass, refractive index, stiffness, membrane tension) at the nanometer scale using optical phase.
Achieved throughput significantly higher than conventional imaging flow cytometry.

Conclusions:

Optical time-stretch microscopy offers a transformative solution for high-throughput, high-content single-cell analysis.
The platform's ability to provide morphological and quantitative data complements existing flow cytometry methods.
This technology is poised to expand the parameter space for big-data driven biomedical research.