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Quantitation of Chromosome Damage by Imaging Flow Cytometry
Lindsay A Beaton-Green1, Ruth C Wilkins2
1Radiobiology, Consumer and Clinical Radiation Protection Bureau, Health Canada, 775 Brookfield Rd., Ottawa, ON, Canada. Lindsay.Beaton@hc-sc.gc.ca.
Methods in Molecular Biology (Clifton, N.J.)
|July 28, 2016
Summary
Rapid biodosimetry using chromosome analysis is crucial for mass casualty events. Adapting the dicentric chromosome assay for imaging flow cytometry enhances throughput for faster radiation dose assessment in exposed individuals.
Area of Science:
- Radiation biology
- Cytogenetics
- Medical countermeasures
Background:
- Biodosimetry assesses radiation exposure using biological markers like chromosome damage.
- Accurate and rapid dose estimation is vital for medical management after mass casualty incidents.
- The dicentric chromosome assay is the established method for biodosimetry.
Purpose of the Study:
- To adapt the dicentric chromosome assay for high-throughput analysis.
- To enable faster radiation dose assessment in mass casualty scenarios.
- To describe methods for quantifying chromosomal aberrations using imaging flow cytometry.
Main Methods:
- Utilizing imaging flow cytometry for chromosome analysis.
- Adapting the dicentric chromosome assay for automated detection.
- Developing methods for identifying and quantifying mono- and multicentric chromosomes.
Main Results:
- The imaging flow cytometry adaptation allows for increased analysis throughput.
- The method enables efficient identification of radiation-induced chromosomal damage.
- Quantification of specific chromosome types (mono- and multicentric) is achieved.
Conclusions:
- Imaging flow cytometry offers a scalable solution for biodosimetry.
- This adapted assay can significantly improve response times in mass casualty events.
- The described methods provide a foundation for rapid radiation dose assessment.
