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Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

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The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: Mar 17, 2026

A Precision Medicine Tool for Measurement and Monitoring of Hemoglobin S in Sickle Cell Disease Patients Receiving Transfusion Therapy
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Sickle Cell Imaging Flow Cytometry Assay (SIFCA).

Kleber Y Fertrin1,2, Leigh Samsel3, Eduard J van Beers4

  • 1Department of Clinical Pathology, School of Medical Sciences, University of Campinas, Campinas, Brazil.

Methods in Molecular Biology (Clifton, N.J.)
|July 28, 2016
PubMed
Summary
This summary is machine-generated.

Sickle cell disorders involve red blood cells changing shape due to hemoglobin S. A new imaging flow cytometry assay offers a faster, automated way to test potential sickle cell disorder treatments.

Keywords:
Imaging flow cytometrySickle cell diseaseSickling assay

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Area of Science:

  • Hematology
  • Cell Biology
  • Medical Diagnostics

Background:

  • Sickle cell disorders are characterized by hemoglobin S polymerization, leading to red blood cell sickling under hypoxia.
  • Current methods for assessing anti-sickling agents are labor-intensive and subjective, hindering drug discovery.
  • Developing efficient assays is crucial for identifying novel therapeutic strategies for sickle cell disorders.

Purpose of the Study:

  • To develop and validate a partially automated, high-throughput sickling assay.
  • To improve the efficiency and objectivity of evaluating agents that inhibit red blood cell sickling.
  • To facilitate the screening of potential therapeutics for sickle cell disorders.

Main Methods:

  • Utilized imaging flow cytometry for automated red blood cell imaging and analysis.
  • Developed protocols for sample preparation and data acquisition in a high-throughput format.
  • Validated the assay's performance against established sickling assessment methods.

Main Results:

  • The imaging flow cytometry assay demonstrated high throughput capabilities.
  • The assay provided objective and reproducible measurements of red blood cell sickling.
  • The developed method significantly reduced assay time and observer dependency compared to traditional methods.

Conclusions:

  • A partially automated, high-throughput sickling assay using imaging flow cytometry is effective.
  • This novel assay streamlines the evaluation of anti-sickling agents.
  • The assay represents a significant advancement for sickle cell disorder research and therapeutic development.