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Microarrays/DNA Chips for the Detection of Waterborne Pathogens
1Host-Pathogen Unit, Research Institute for Medicine (iMed-ULisboa), Faculdade de Farmácia da Universidade de Lisboa, Universidade de Lisboa, Lisbon, Portugal. f.vale@ff.ul.pt.
Methods in Molecular Biology (Clifton, N.J.)
|July 28, 2016
Abstract:
DNA microarrays are useful for the simultaneous detection of microorganisms in water samples. Specific probes targeting waterborne pathogens are selected with bioinformatics tools, synthesized and spotted onto a DNA array. Here, the construction of a DNA chip for waterborne pathogen detection is described, including the processes of probe in silico selection, synthesis, validation, and data analysis.

