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Stemphyltoxin III from Alternaria alternata.

M E Stack¹, E P Mazzola

¹Division of Contaminants Chemistry, Food and Drug Administration, Washington, D.C. 20204.

Journal of Natural Products

|March 1, 1989

Summary

Stemphyltoxin III, a known metabolite from Stemphylium botryosum, was identified as a mutagenic compound produced by Alternaria alternata. This study presents its previously unreported 13C-NMR spectral data.

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Area of Science:

Natural Product Chemistry
Mycotoxicology
Spectroscopic Analysis

Background:

Stemphyltoxin III is a known metabolite of *Stemphylium botryosum var. lactucum*.
Metabolites from fungal species can possess significant biological activities, including mutagenicity.
Identification of novel toxic compounds is crucial for risk assessment.

Purpose of the Study:

To identify and characterize mutagenic metabolites produced by *Alternaria alternata*.
To report the 13C-NMR spectral data of Stemphyltoxin III, which were previously unreported.
To confirm Stemphyltoxin III as a mutagenic metabolite of *Alternaria alternata*.

Main Methods:

Spectroscopic studies, including 13C-NMR, were employed for structural elucidation.
Comparative analysis of spectral data with known compounds.
Isolation and purification of the metabolite from fungal cultures.

Main Results:

Stemphyltoxin III, previously identified in *S. botryosum*, was confirmed as a metabolite of *A. alternata*.
The compound was identified as mutagenic.
Complete 13C-NMR spectral data for Stemphyltoxin III are presented for the first time.

Conclusions:

Stemphyltoxin III is a mutagenic metabolite produced by *Alternaria alternata*.
The characterization and spectral data contribute to the understanding of fungal secondary metabolites.
This finding has implications for food safety and agricultural practices due to potential contamination.

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