Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Phosphoinositides and PIPs01:42

Phosphoinositides and PIPs

10.5K
Phosphoinositides are a group of phospholipids containing a glycerol backbone with two fatty acid chains and a phosphate attached to a myoinositol sugar ring. The inositol head group extends into the cytoplasm, where it is modified by adding phosphate groups to form phosphatidylinositol phosphates or PIPs.
Different phosphoinositides are synthesized and recruited on the cytosolic face of the plasma membrane. The localization of specific phosphoinositides concentrated in separate membrane...
10.5K
Immunoprecipitation01:20

Immunoprecipitation

7.8K
Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...
7.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Proteome-wide quantification of inositol pyrophosphate-protein interactions.

Nature communications·2026
Same author

<i>Lotus japonicus</i> VIH2 is an inositol pyrophosphate synthase that regulates arbuscular mycorrhiza.

Science advances·2026
Same author

Protein pyrophosphorylation by inositol pyrophosphates - detection, function, and regulation.

FEBS letters·2025
Same author

NUDIX hydrolases target specific inositol pyrophosphates and regulate phosphate homeostasis and bacterial pathogen susceptibility in Arabidopsis.

Journal of integrative plant biology·2025
Same author

Enabling Technologies for the Dissection of Inositol Pyrophosphate Physiology.

Methods in molecular biology (Clifton, N.J.)·2025
Same author

Enzymatic Synthesis of Inositol Pyrophosphates.

Methods in molecular biology (Clifton, N.J.)·2025

Related Experiment Video

Updated: Mar 17, 2026

Preparation of Quality Inositol Pyrophosphates
10:34

Preparation of Quality Inositol Pyrophosphates

Published on: September 3, 2011

13.7K

Chemical tools for interrogating inositol pyrophosphate structure and function.

Nathaniel W Brown1, Alan M Marmelstein1, Dorothea Fiedler1

  • 1Princeton University, Frick Chemistry Laboratory, Washington Road, Princeton, NJ 08544, USA and Leibniz-Institut für Molekulare Pharmakologie, Robert-Rössle-Str 10, 13125 Berlin, Berlin, Germany. dfiedler@fmp-berlin.de.

Chemical Society Reviews
|July 28, 2016
PubMed
Summary

Inositol pyrophosphates (PP-InsPs) are vital cellular messengers. Recent advances in synthesis and detection methods are clarifying their roles in cell signaling and energy balance.

More Related Videos

Absolute Quantitation of Inositol Pyrophosphates by Capillary Electrophoresis Electrospray Ionization Mass Spectrometry
09:22

Absolute Quantitation of Inositol Pyrophosphates by Capillary Electrophoresis Electrospray Ionization Mass Spectrometry

Published on: August 13, 2021

2.8K
Extraction and Quantification of Soluble, Radiolabeled Inositol Polyphosphates from Different Plant Species using SAX-HPLC
09:01

Extraction and Quantification of Soluble, Radiolabeled Inositol Polyphosphates from Different Plant Species using SAX-HPLC

Published on: June 26, 2020

7.6K

Related Experiment Videos

Last Updated: Mar 17, 2026

Preparation of Quality Inositol Pyrophosphates
10:34

Preparation of Quality Inositol Pyrophosphates

Published on: September 3, 2011

13.7K
Absolute Quantitation of Inositol Pyrophosphates by Capillary Electrophoresis Electrospray Ionization Mass Spectrometry
09:22

Absolute Quantitation of Inositol Pyrophosphates by Capillary Electrophoresis Electrospray Ionization Mass Spectrometry

Published on: August 13, 2021

2.8K
Extraction and Quantification of Soluble, Radiolabeled Inositol Polyphosphates from Different Plant Species using SAX-HPLC
09:01

Extraction and Quantification of Soluble, Radiolabeled Inositol Polyphosphates from Different Plant Species using SAX-HPLC

Published on: June 26, 2020

7.6K

Area of Science:

  • Biochemistry
  • Cellular Biology
  • Molecular Signaling

Background:

  • Inositol pyrophosphates (PP-InsPs) are highly phosphorylated intracellular messengers involved in cellular energy homeostasis and signal transduction.
  • Despite over two decades of research, challenges in identifying isomers and biochemical pathways have hindered a full understanding of PP-InsP function.
  • Key questions remain regarding physiologically relevant isomers, their associated phenotypes, and signal transduction mechanisms.

Approach:

  • Total synthesis of various PP-InsP isomers has been crucial for advancing research.
  • Development of new methods to identify downstream signaling partners has facilitated progress.
  • Chemical tools have been created to differentiate between proposed PP-InsP signaling mechanisms, including protein binding and protein pyrophosphorylation.

Key Points:

  • Recent synthetic and analytical advancements are enabling deeper insights into PP-InsP structure and function.
  • Understanding PP-InsPs is critical for deciphering cellular energy homeostasis and complex signaling networks.
  • New chemical tools are helping to elucidate the distinct modes of PP-InsP signal transduction.

Conclusions:

  • Technical challenges in studying PP-InsPs are being overcome by innovative synthetic and detection strategies.
  • These advancements are crucial for understanding the fundamental roles of PP-InsPs in cellular processes.
  • Future research opportunities lie in further exploring PP-InsP signaling pathways and their therapeutic potential.