Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Phosphoinositides and PIPs01:42

Phosphoinositides and PIPs

8.7K
Phosphoinositides are a group of phospholipids containing a glycerol backbone with two fatty acid chains and a phosphate attached to a myoinositol sugar ring. The inositol head group extends into the cytoplasm, where it is modified by adding phosphate groups to form phosphatidylinositol phosphates or PIPs.
Different phosphoinositides are synthesized and recruited on the cytosolic face of the plasma membrane. The localization of specific phosphoinositides concentrated in separate membrane...
8.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Fasting disrupts the InsP₆-HDAC3 axis to drive ER stress-mediated clearance of DNA-damaged cells and enforce tissue quality control.

bioRxiv : the preprint server for biology·2026
Same author

Inositol biosynthesis is inversely regulated by glycolytic activity.

Biochimica et biophysica acta. Molecular and cell biology of lipids·2026
Same author

Proteome-wide quantification of inositol pyrophosphate-protein interactions.

Nature communications·2026
Same author

<i>Lotus japonicus</i> VIH2 is an inositol pyrophosphate synthase that regulates arbuscular mycorrhiza.

Science advances·2026
Same author

How interdiction of inositol pyrophosphate catabolism perturbs the fission yeast response to phosphate starvation.

Nucleic acids research·2026
Same author

Pentaphosphorylated magic spot nucleotides: chemoenzymatic synthesis and disassembly-based sensing.

Organic & biomolecular chemistry·2026

Related Experiment Video

Updated: Sep 9, 2025

Absolute Quantitation of Inositol Pyrophosphates by Capillary Electrophoresis Electrospray Ionization Mass Spectrometry
09:22

Absolute Quantitation of Inositol Pyrophosphates by Capillary Electrophoresis Electrospray Ionization Mass Spectrometry

Published on: August 13, 2021

2.4K

Enabling Technologies for the Dissection of Inositol Pyrophosphate Physiology.

Adolfo Saiardi1, Henning J Jessen2, Dorothea Fiedler3

  • 1Laboratory for Molecular Cell Biology, University College London, London, UK. a.saiardi@ucl.ac.uk.

Methods in Molecular Biology (Clifton, N.J.)
|August 29, 2025
PubMed
Summary

Inositol pyrophosphates are crucial cell messengers. New experimental methods are overcoming challenges in studying these molecules, advancing research into their diverse roles.

Keywords:
Allosteric bindingCapillary electrophoresisExperimental modelHistorical perspectiveLiquid chromatographyMass spectrometryMetabolismNuclear magnetic resonancePyrophosphorylationSignaling

More Related Videos

Preparation of Quality Inositol Pyrophosphates
10:34

Preparation of Quality Inositol Pyrophosphates

Published on: September 3, 2011

13.2K
Extraction and Quantification of Soluble, Radiolabeled Inositol Polyphosphates from Different Plant Species using SAX-HPLC
09:01

Extraction and Quantification of Soluble, Radiolabeled Inositol Polyphosphates from Different Plant Species using SAX-HPLC

Published on: June 26, 2020

7.1K

Related Experiment Videos

Last Updated: Sep 9, 2025

Absolute Quantitation of Inositol Pyrophosphates by Capillary Electrophoresis Electrospray Ionization Mass Spectrometry
09:22

Absolute Quantitation of Inositol Pyrophosphates by Capillary Electrophoresis Electrospray Ionization Mass Spectrometry

Published on: August 13, 2021

2.4K
Preparation of Quality Inositol Pyrophosphates
10:34

Preparation of Quality Inositol Pyrophosphates

Published on: September 3, 2011

13.2K
Extraction and Quantification of Soluble, Radiolabeled Inositol Polyphosphates from Different Plant Species using SAX-HPLC
09:01

Extraction and Quantification of Soluble, Radiolabeled Inositol Polyphosphates from Different Plant Species using SAX-HPLC

Published on: June 26, 2020

7.1K

Area of Science:

  • Biochemistry
  • Cell Signaling
  • Molecular Biology

Background:

  • Inositol pyrophosphates (IPs) are a class of small molecules with diverse chemical structures.
  • IPs function as critical signaling molecules in various cellular processes.
  • Studying IPs is challenging due to their high charge, lack of detectable markers, structural similarity, and complex metabolism.

Purpose of the Study:

  • To review the challenges in inositol pyrophosphate research.
  • To summarize recent experimental advances enabling IPs studies.
  • To highlight the future directions in the field of inositol pyrophosphate signaling.

Main Methods:

  • Literature review of experimental techniques for IPs analysis.
  • Discussion of novel detection and quantification strategies.
  • Overview of metabolic pathway elucidation approaches.

Main Results:

  • Several experimental strategies have been developed to overcome the inherent difficulties in studying IPs.
  • Recent advances facilitate the characterization of IPs' structures and functions.
  • The field is progressing towards a deeper understanding of IPs' roles in cell signaling.

Conclusions:

  • Despite significant challenges, innovative experimental approaches are transforming inositol pyrophosphate research.
  • Future studies will likely uncover more about the complex roles of these signaling molecules.
  • Advancements promise to propel the field forward, revealing new insights into cellular regulation.