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Updated: Mar 17, 2026

Expression of Exogenous Cytokine in Patient-derived Xenografts via Injection with a Cytokine-transduced Stromal Cell Line

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P-Glycoprotein Expression in Drug-Resistant Chronic Lymphoproliferative Disorder.

G Majumdar¹, A K Singh¹

¹a Division of Haematology, United Medical and Dental School of Guy's and St Thomas', St Thomas' Campus, London, England.

Leukemia & Lymphoma

|July 28, 2016

Summary

P-glycoprotein (P-gp) expression was studied in drug-resistant chronic lymphoproliferative disorders. P-gp was found in Adult T-cell leukemia/lymphoma and some prolymphocytic leukemia and chronic lymphocytic leukemia patients, suggesting a role in multidrug resistance.

Keywords:

P-Glycoprotein chronic lymphocytic leukaemia lymphoproliferative disorders prolymphocytic leukaemia

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Subcellular Fractionation of Primary Chronic Lymphocytic Leukemia Cells to Monitor Nuclear/Cytoplasmic Protein Trafficking

Subcellular Fractionation of Primary Chronic Lymphocytic Leukemia Cells to Monitor Nuclear/Cytoplasmic Protein Trafficking

Published on: October 23, 2019

Area of Science:

Hematology
Oncology
Molecular Biology

Background:

Chronic lymphoproliferative disorders (LPD) often exhibit drug resistance.
P-glycoprotein (P-gp) is a key mediator of multidrug resistance (MDR) in cancer cells.
Understanding P-gp expression in LPD is crucial for developing effective treatment strategies.

Purpose of the Study:

To investigate the expression of P-glycoprotein (P-gp) in peripheral blood lymphocytes of patients with drug-resistant chronic lymphoproliferative disorders (LPD).
To determine the potential role of P-gp in mediating multidrug resistance in various subtypes of LPD.

Main Methods:

Direct immunofluorescence (IF) testing using monoclonal antibody (MoAb) C219 was employed.
Peripheral blood lymphocytes from 60 patients with drug-resistant LPD were analyzed.
Specific LPD subtypes included prolymphocytic leukemia (PLL), Adult T-cell leukemia/lymphoma (ATLL), hairy cell leukemia (HCL), and chronic lymphocytic leukemia (CLL).

Main Results:

P-gp expression was detected in 4/15 PLL patients and both ATLL patients.
None of the HCL patients expressed P-gp.
P-gp was found in 2/14 anthracycline-resistant CLL patients, but not in CLL patients resistant only to chlorambucil.
P-gp expression levels varied significantly (20-100%) among positive cases.
PMA stimulation did not induce P-gp in non-resistant CLL lymphocytes.

Conclusions:

Multidrug resistance in ATLL is likely mediated by P-gp over-expression.
P-gp may also play a significant role in multidrug resistance in a subset of PLL and CLL patients.
These findings highlight P-gp as a potential therapeutic target in specific drug-resistant lymphoproliferative disorders.