Related Experiment Video
Updated: Mar 17, 2026
11:48
Generation, Amplification, and Titration of Recombinant Respiratory Syncytial Viruses
Published on: April 4, 2019
17.5K
Reverse Genetics of Respiratory Syncytial Virus
Christopher C Stobart1,2, Anne L Hotard1,2, Jia Meng1,2
1Department of Pediatrics, Emory University School of Medicine, Atlanta, GA, 30322, USA.
Methods in Molecular Biology (Clifton, N.J.)
|July 29, 2016
Summary
Researchers developed new methods to recover recombinant Respiratory Syncytial Virus (RSV) using bacterial artificial chromosomes. This advancement aids in studying RSV mutants, crucial for developing urgently needed vaccines against this common infant respiratory illness.
Area of Science:
- Virology
- Molecular Biology
- Vaccine Development
Background:
- Respiratory Syncytial Virus (RSV) causes severe lower respiratory tract infections, particularly in infants and the elderly.
- RSV is a major global cause of infant mortality, highlighting a significant unmet medical need.
- Currently, no vaccines are available for RSV, despite its substantial clinical and economic impact.
Purpose of the Study:
- To describe methods for the recovery of recombinant RSV using a bacterial artificial chromosome (BAC).
- To outline procedures for obtaining and expanding stocks of RSV mutants.
- To provide foundational techniques for future RSV vaccine and therapeutic research.
Main Methods:
- Utilized a bacterial artificial chromosome (BAC) system for the recovery of infectious recombinant RSV.
- Developed protocols for the procurement and subsequent expansion of viral stocks.
- Employed molecular biology techniques for the generation and manipulation of RSV mutants.
Main Results:
- Successfully recovered infectious recombinant RSV viruses through the described BAC-based method.
- Established reliable procedures for the procurement and expansion of RSV mutant stocks.
- Demonstrated the feasibility of using these methods for generating and handling RSV genetic variants.
Conclusions:
- The described BAC-based recovery system provides a robust platform for generating recombinant RSV.
- These methods facilitate the study and manipulation of RSV mutants, essential for understanding viral pathogenesis.
- This work lays the groundwork for developing novel vaccines and antiviral strategies against RSV.
Keywords:
Bacterial artificial chromosomeRSVRespiratory syncytial virusReverse geneticsVirus recoveryMore Related Videos
Related Concept Videos
01:03Rous Sarcoma Virus (RSV) and Cancer
6.5K
Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...
6.5K
02:33Retroviruses
15.6K
Retroviruses and retrotransposons both insert copies of their genetic elements into the genome of the host cell. Thus, the viral genes are passed on when the host genome is replicated or translated. A typical retroviral DNA sequence contains 3-4 genes that encode the different proteins required for its structural assembly and function as a molecular parasite. This DNA is transcribed into a single mRNA, which is very similar in structure to conventional mRNAs, i.e., it is capped at the 5’...
15.6K
01:10Retrovirus Life Cycles
50.3K
Retroviruses have a single-stranded RNA genome that undergoes a special form of replication. Once the retrovirus has entered the host cell, an enzyme called reverse transcriptase synthesizes double-stranded DNA from the retroviral RNA genome. This DNA copy of the genome is then integrated into the host’s genome inside the nucleus via an enzyme called integrase. Consequently, the retroviral genome is transcribed into RNA whenever the host’s genome is transcribed, allowing the...
50.3K
01:29Viruses with RNA Genomes
1.2K
RNA viruses are categorized into positive-strand, negative-strand, or double-stranded groups based on their genomic structure and replication mechanisms. This classification dictates how they exploit host cellular machinery for protein synthesis and replication. Some RNA viruses also utilize reverse transcription as part of their life cycle, further diversifying their replication strategies.Positive-Strand RNA VirusesPositive-strand RNA viruses have genomes that function directly as messenger...
1.2K
02:32Exon Recombination
4.3K
The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes.
Exon shuffling follows “splice frame rules.” Each exon...
Exon shuffling follows “splice frame rules.” Each exon...
4.3K
00:36Viral Mutations
40.6K
A mutation is a change in the sequence of bases of DNA or RNA in a genome. Some mutations occur during replication of the genome due to errors made by the polymerase enzymes that replicate DNA or RNA. Unlike DNA polymerase, RNA polymerase is prone to errors because it is not capable of “proofreading” its work. Viruses with RNA-based genomes, like HIV, therefore accrue mutations faster than viruses with DNA-based genomes. Because mutation and recombination provide the raw material...
40.6K