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Related Experiment Video

Updated: Mar 17, 2026

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment
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Fast, label-free super-resolution live-cell imaging using rotating coherent scattering (ROCS) microscopy.

Felix Jünger1, Philipp V Olshausen1,2, Alexander Rohrbach1,3

  • 1Laboratory for Bio- and Nano-Photonics, Department of Microsystems Engineering, University of Freiburg, Germany.

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|July 29, 2016
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Summary

This study introduces a novel label-free microscopy method for imaging dynamic cellular structures. The technique achieves super-resolution at high speeds without post-processing, revealing previously unseen cellular activities.

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Area of Science:

  • Cell Biology
  • Microscopy
  • Biophysics

Background:

  • Living cells exhibit dynamic behavior, with structures often below the resolution limit of conventional microscopes.
  • Existing super-resolution microscopy techniques typically rely on fluorescence labeling and are too slow for dynamic processes.
  • Label-free imaging is desirable for live-cell studies to avoid perturbation from exogenous labels.

Purpose of the Study:

  • To develop a label-free microscopy technique capable of high-speed, super-resolution imaging of dynamic cellular structures.
  • To overcome limitations of existing super-resolution methods in terms of speed and the need for cell labeling.
  • To visualize subcellular dynamics in living cells without post-processing.

Main Methods:

  • Utilized oblique sample illumination with coherent light and a 360° circulating laser beam.
  • Combined total internal reflection illumination with dark-field detection.
  • Leveraged local destructive interferences to enhance contrast and resolution.
  • Detected local changes in refractive index via scattered laser light.

Main Results:

  • Achieved super-resolved, high-contrast images at a frame rate of 100 Hertz without post-processing.
  • Resolved structures as small as 150 nm.
  • Successfully applied the technique to living mouse macrophages and helical bacteria.
  • Observed unexpected dynamic processes within these living samples.

Conclusions:

  • The developed label-free microscopy technique offers a powerful new tool for studying live-cell dynamics at the nanoscale.
  • This method overcomes the speed and labeling limitations of traditional super-resolution microscopy.
  • The ability to image refractive index changes reveals dynamic cellular events previously inaccessible.