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[Activation of complement by hemodialysis membrane].

H Kojima, H Ohi, M Seki

    Nihon Jinzo Gakkai Shi
    |January 1, 1989
    PubMed
    Summary

    Hemodialysis membranes activate the complement system, primarily via the alternative pathway. This process generates terminal complement complex (TCC) and anaphylatoxins, indicated by increased iC3b and Bb levels in patients.

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    Diabetologia·1998

    Area of Science:

    • Immunology
    • Nephrology

    Context:

    • Hemodialysis is a vital treatment for kidney failure.
    • The interaction between hemodialysis membranes and the complement system is not fully understood.
    • Complement activation can lead to adverse effects during hemodialysis.

    Purpose:

    • To investigate the in vitro and in vivo activation of the human complement system by hemodialysis membranes.
    • To identify the specific complement pathway involved in membrane-induced activation.
    • To measure the levels of complement activation products during hemodialysis in polycystic kidney disease patients.

    Summary:

    • Incubation of human serum with hemodialysis membranes induced complement component C3 conversion and C3d formation in vitro.
    • ELISA detected increased levels of complement breakdown products iC3b and Bb in plasma from hemodialysis patients, while C4d levels remained unchanged.

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  • Terminal complement complex (TCC) was detected after membrane incubation in vitro and in patient plasma, suggesting alternative pathway activation.

    • Impact:

    • Findings suggest hemodialysis membranes predominantly activate the complement system through the alternative pathway.
    • The formation of TCC and anaphylatoxins during hemodialysis may have clinical implications.
    • This study provides insights into the immunological consequences of hemodialysis membrane-complement interactions.