Related Experiment Video

Updated: Mar 17, 2026

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells
11:35

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells

Published on: June 16, 2017

13.4K

An episomal CRISPR/Cas9 system to derive vector-free gene modified mammalian cells

Linlin Li1, Fei Gao1, Sen Wu2

  • 1State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, 100193, China.

Protein & Cell
|July 31, 2016
PubMed
Abstract

No abstract available in PubMed .

More Related Videos

CRISPR-Cas9 Mediated Gene Deletion in Human Pluripotent Stem Cells Cultured Under Feeder-Free Conditions
04:21

CRISPR-Cas9 Mediated Gene Deletion in Human Pluripotent Stem Cells Cultured Under Feeder-Free Conditions

Published on: November 1, 2024

1.4K
Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9
09:40

Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9

Published on: January 3, 2015

96.8K

Related Experiment Videos

Last Updated: Mar 17, 2026

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells
11:35

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells

Published on: June 16, 2017

13.4K
CRISPR-Cas9 Mediated Gene Deletion in Human Pluripotent Stem Cells Cultured Under Feeder-Free Conditions
04:21

CRISPR-Cas9 Mediated Gene Deletion in Human Pluripotent Stem Cells Cultured Under Feeder-Free Conditions

Published on: November 1, 2024

1.4K
Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9
09:40

Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9

Published on: January 3, 2015

96.8K

Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

2.4K
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
2.4K
CRISPR01:59

CRISPR

58.7K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
58.7K

Articles linked to this work by shared authors, journal, and citation graph.

The glutathione peroxidase gene family in Thellungiella salsuginea: genome-wide identification, classification, and gene and protein expression analysis under stress conditions.

International journal of molecular sciences·2014

Genetic manipulation of a transcription-regulating sequence of porcine reproductive and respiratory syndrome virus reveals key nucleotides determining its activity.

Archives of virology·2014

Two monoclonal antibodies recognising aa 634-668 and aa 1026-1055 of NogoA enhance axon extension and branching in cultured neurons.

PloS one·2014

Preparation and characterization of starch crosslinked with sodium trimetaphosphate and hydrolyzed by enzymes.

Carbohydrate polymers·2014

Energetics of defects on graphene through fluorination.

ChemSusChem·2014

Incidence of microscopically positive proximal margins in adenocarcinoma of the gastroesophageal junction.

PloS one·2014

Complement-dependent phagocytosis in the clearance of various cellular targets.

Protein & cell·2026

O-GlcNAc clusters in intrinsically disordered regions regulate transcriptional condensates and gene control.

Protein & cell·2026

CRISPR screening identifies GNPTAB as a noncanonical STING activator driving cellular senescence.

Protein & cell·2026

Shared CRISPR arrays underpin type I-A/I-B coexistence.

Protein & cell·2026

Lipid droplets, an enigmatic organelle under the spotlight.

Protein & cell·2026

African swine fever virus MGF_110-3L and MGF_110-4L signal TLR2-mediated lethal inflammation.

Protein & cell·2026

Recent advances in electrocatalytic urea synthesis via N2 and CO2 coupling: mechanisms, catalyst design and reactor engineering.

Chemical communications (Cambridge, England)·2026

Photochemical functionalisation of cysteine using [1.1.1]propellane.

Chemical communications (Cambridge, England)·2026

Radiofrequency Microneedling Combined With Topical Extracellular Vesicle Preparation for Facial Rejuvenation: A Randomized Split-Face Controlled Study.

The Journal of craniofacial surgery·2026

Unlocking anammox potential: functional group-optimized carbon quantum dots enhance nitrogen removal via multi-pathway metabolic synergy.

Bioresource technology·2026

Total Synthesis of D-Fucp3NAc Containing Conjugation-Ready Pentasaccharide Repeating Unit Related to Acinetobacter baumannii RCH51.

The Journal of organic chemistry·2026

Synthetic Studies toward Neotatarine.

The Journal of organic chemistry·2026
See all related articles
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies
Jove
Visualize
Contact Us