Related Experiment Video
Updated: Mar 17, 2026
09:22
Ultrafast Lignin Extraction from Unusual Mediterranean Lignocellulosic Residues
Published on: March 9, 2021
7.7K
Lignin profiling in extracted xylans by size-exclusion chromatography
Christian Hutterer1, Gabriele Schild2, Gerhard Kliba2
1Kompetenzzentrum Holz GmbH, Altenberger Str. 69, A-4040 Linz, Austria.
Carbohydrate Polymers
|July 31, 2016
Summary
This study presents a new method for analyzing lignin in xylan-rich streams from biorefineries. The technique uses size-exclusion chromatography and UV detection to profile residual lignin, aiding in the characterization of novel xylan products.
Area of Science:
- Biorefinery technologies
- Polymer chemistry
- Analytical chemistry
Background:
- Lignocellulose utilization is key for future biorefineries.
- Hemicelluloses, particularly xylans, are valuable components.
- Alkaline extraction for hemicellulose also extracts residual lignin.
Purpose of the Study:
- To develop a method for profiling lignin in xylan-containing alkaline lyes.
- To visualize and quantify residual lignin distribution.
- To enable advanced characterization of novel xylan products.
Main Methods:
- Hyphenation of size-exclusion chromatography (SEC) with UV detection.
- Utilizing lignin standards prepared from Kraft cooking liquors for quantification.
- Analysis of hemicelluloses and co-extracted lignins from wood species.
Main Results:
- Successful setup of a lignin profiling method.
- Visualization of residual lignin distribution in xylan streams.
- Simultaneous quantification of lignin achieved.
Conclusions:
- The presented method allows for detailed characterization of lignin in xylan products.
- This analytical approach supports the development of advanced biorefinery processes.
- Improved understanding of lignin-xylan interactions in alkaline pulping.
