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Updated: Mar 17, 2026

Microplot Design and Plant and Soil Sample Preparation for 15Nitrogen Analysis
Published on: May 10, 2020
δ(15) N from soil to wine in bulk samples and proline
Mauro Paolini1,2, Luca Ziller1, Daniela Bertoldi1
1Fondazione Edmund Mach (FEM), Via E. Mach 1, 38010 San Michele all'Adige, Trento, Italy.
Investigating nitrogen-15 isotope ratios (δ(15)N) in wine reveals its potential for geographical traceability. Wine proline
Area of Science:
- Agricultural Chemistry
- Food Science
- Isotope Geochemistry
Background:
- Geographical origin is a key factor in wine quality and value.
- Traditional traceability methods can be complex and costly.
- Nitrogen isotope ratios (δ(15)N) offer a potential new marker for origin tracing.
Purpose of the Study:
- To assess the feasibility of using δ(15)N as an isotopic marker for wine's geographical origin.
- To investigate the entire soil-grape-wine production chain.
- To evaluate the impact of various winemaking processes on δ(15)N values.
Main Methods:
- Analysis of δ(15)N in soil, leaves, grapes, and wine (bulk and proline).
- Studying the effects of fermentation, yeast type, vinification (red/white), nitrogen adjuvants, and ultrasound lysis.
- First-time measurement of δ(15)N in grape must and wine proline.
Main Results:
- δ(15)N values in leaves, grapes, and wine, especially proline, reflect soil δ(15)N variability.
- Fermentation and ultrasound treatments did not alter grape must δ(15)N.
- Adjuvant addition affected bulk wine δ(15)N, but not wine proline δ(15)N.
Conclusions:
- Wine proline δ(15)N is a robust marker for tracing wine's geographical origin.
- Nitrogen isotope analysis, particularly of proline, offers a reliable method for wine authentication.
- This isotopic marker can help ensure wine authenticity and protect regional designations.
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