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Safety considerations derived from Cry34Ab1/Cry35Ab1 structure and function.

Kenneth E Narva1, Nick X Wang1, Rod Herman1

  • 1Dow AgroSciences, 9330 Zionsville Road, Indianapolis, IN 46268, USA.

Journal of Invertebrate Pathology
|August 3, 2016
PubMed
Summary
This summary is machine-generated.

Safety testing of insecticidal proteins like Bacillus thuringiensis (Bt) Cry34Ab1 and Cry35Ab1 in crops is crucial. Their unique structures, distinct from other Bt proteins, are examined for safety in insect-resistant maize.

Keywords:
Bacillus thuringiensisBt cropsCry toxinCry34Ab1Cry35Ab1Three-domain Cry toxin

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Area of Science:

  • Agricultural Science
  • Molecular Biology
  • Biochemistry

Background:

  • Insecticidal proteins, such as Bacillus thuringiensis (Bt) Cry34Ab1 and Cry35Ab1, are developed for crop protection against pests like the western corn rootworm.
  • Safety assessments are integral to the development of insect-resistant crops, adhering to science-based guidelines.
  • The protein structures of Cry34Ab1 and Cry35Ab1 differ from commonly used insecticidal proteins in maize.

Purpose of the Study:

  • To review the structure and function of Cry34Ab1 and Cry35Ab1 proteins.
  • To contextualize these proteins within safety studies for insect-resistant crops.
  • To highlight the unique structural characteristics of Cry34Ab1 and Cry35Ab1.

Main Methods:

  • Analysis of protein crystal structures of Cry34Ab1 and Cry35Ab1.
  • Comparison of Cry34Ab1/Cry35Ab1 structures with known insecticidal proteins (e.g., three-domain Cry, Vip3, B. sphaericus BinA/BinB).
  • Evaluation of structural similarities to membrane-active proteins and pore-forming proteins.

Main Results:

  • Cry34Ab1 exhibits a single-domain beta sandwich structure, similar to cytolysins.
  • Cry35Ab1 possesses two domains, one with sugar-binding motifs and another related to beta pore-forming proteins.
  • These structural features distinguish Cry34Ab1/Cry35Ab1 from other Bt insecticidal proteins.

Conclusions:

  • The distinct structural characteristics of Cry34Ab1 and Cry35Ab1 are important considerations in their safety evaluation for insect-resistant maize.
  • Understanding protein structure provides insights into potential function and safety profiles.
  • Case-by-case safety studies are essential for insecticidal proteins used in crop development.