Related Experiment Video
Updated: Mar 16, 2026
07:55
Analyzing Cell Surface Adhesion Remodeling in Response to Mechanical Tension Using Magnetic Beads
Published on: March 8, 2017
9.0K
NBR1-dependent selective autophagy is required for efficient cell-matrix adhesion site disassembly
Candia M Kenific1,2, Jayanta Debnath1
1a Department of Pathology and Helen Diller Family Comprehensive Cancer Center , University of California San Francisco , San Francisco , CA , USA.
Autophagy
|August 4, 2016
Summary
Selective autophagy, a cellular process, aids cell migration by degrading focal adhesions. The NBR1 protein targets these adhesion sites for breakdown, revealing a new role for autophagy in cell biology.
Area of Science:
- Cell Biology
- Molecular Biology
- Autophagy Research
Background:
- Macroautophagy/autophagy is crucial for cellular survival under stress.
- Selective autophagy regulates cellular homeostasis by degrading specific contents.
- Recent findings suggest autophagy impacts diverse cell biological processes.
Purpose of the Study:
- To investigate the role of selective autophagy in cell migration.
- To identify the mechanism by which selective autophagy influences focal adhesions.
- To determine the involvement of autophagy cargo receptors in this process.
Main Methods:
- Utilized cell migration assays.
- Investigated the turnover of focal adhesions.
- Examined the function of the autophagy cargo receptor NBR1.
- Analyzed the targeting of autophagosomes to focal adhesions.
Main Results:
- Selective autophagy promotes cell migration by facilitating focal adhesion turnover.
- The autophagy cargo receptor NBR1 is essential for targeting autophagosomes to focal adhesions.
- NBR1 mediates the disassembly of focal adhesions through sequestration of their proteins.
- Focal adhesions are identified as a novel cellular target for selective autophagy.
Conclusions:
- Selective autophagy plays a significant role in regulating cell migration.
- NBR1 acts as a key mediator in the selective autophagic degradation of focal adhesions.
- This study reveals focal adhesions as a new class of cargo for selective autophagy, impacting cellular processes beyond stress survival.
Related Concept Videos
01:32Export of Misfolded Proteins out of the ER
5.4K
After folding, the ER assesses the quality of secretory and membrane proteins. The correctly folded proteins are cleared by the calnexin cycle for transport to their final destination, while misfolded proteins are held back in the ER lumen. The ER chaperones attempt to unfold and refold the misfolded proteins but sometimes fail to achieve the correct native conformation. Such terminally misfolded proteins are then exported to the cytosol by ER-associated degradation or ERAD pathway for...
5.4K
01:16Cell Motility through Blebbing
2.7K
Blebs are a type of membrane protrusion formed by the internal hydrostatic pressure of the cytoplasm. Blebs are observed in several cell types, including fibroblasts, immune cells, and single-celled organisms like the amoeba. The primary function of blebs is cell locomotion and apoptosis, but they are also found during necrosis and cell division. The life cycle of a bleb comprises an initiation phase followed by the expansion and retraction phases.
Blebbing Through the Matrix
In multicellular...
Blebbing Through the Matrix
In multicellular...
2.7K
01:24Adherens Junctions
7.4K
Strong contact points between adjacent cells anchor them to each other, forming tissues. Such anchoring junctions are of two types – adherens junctions and desmosomes. Adherens junctions are abundant in tissues such as epithelium and endothelium, forming a continuous zone of adhesion called the adhesion belt. In other tissues, such as heart muscle, they appear as clusters, linking the cells to produce coordinated heart muscle contraction.
Adherens Junctions are Dynamic
Adherens Junctions are Dynamic
7.4K
01:27Autophagy
6.0K
Autophagy is a self-digesting process by which a cell protects itself from threats both within and outside the cell, ranging from abnormal proteins to invading bacteria. In this process, obsolete components of the cell and invading microbes are degraded by hydrolytic enzymes active in an acidic environment of the lysosomal lumen.
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
6.0K
01:17Intracellular Signaling Affects Focal Adhesions
3.8K
Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
Some...
3.8K
01:45ER Retrieval Pathway
5.0K
In the secretory pathway, vesicles transport proteins from one cellular compartment to another in forward transport to deliver the protein to its correct location. Occasionally, misfolded proteins and incorrect proteins escape their original compartments, and a retrieval pathway is used to return the escaped proteins to their original compartment.
The ER uses many checkpoints to prevent the entry of incorrectly folded or a resident protein as cargo onto a transport vesicle. These mechanisms...
The ER uses many checkpoints to prevent the entry of incorrectly folded or a resident protein as cargo onto a transport vesicle. These mechanisms...
5.0K