Effects and synergy of feed ingredients on canine neoplastic cell proliferation
Corri B Levine1, Julie Bayle2, Vincent Biourge2
1Department of Clinical Sciences, Cornell University College of Veterinary Medicine, Veterinary Medical Center C2-009, Ithaca, 14853, NY, USA.
Background:
Adjunctive use of nutraceuticals in human cancer has shown promise, but little work has been done in canine neoplasia. Previous human research has shown that polyphenols and carotenoids can target multiple pathways in vitro and in vivo. These compounds could synergize or antagonize with currently used chemotherapies, either increasing or decreasing the effectiveness of these drugs. Considering the routine and well controlled feeding practices of most dogs, the use of nutraceuticals incorporated into pet food is attractive, pending proof that the extracts are able to improve remission rates. The aim of this study was to examine five feed ingredients for antiproliferative effects, as well as the interaction with toceranib phosphate and doxorubicin hydrochloride, when treating canine neoplastic cell lines in vitro.
Results:
Screening using MTT proliferation assays showed that green tea, turmeric, and rosemary extracts were the most effective. Turmeric extract (TE) was the most potent and exhibited synergy with a rosemary extract (RE) at concentrations from 1 to 25 μg mL(-1). This combination had an additive or synergistic effect with chemotherapeutic agents at selected concentrations within each cell line. No significant effects on cell viability were observed when the combination therapy was used with normal primary cells.
Conclusions:
The use of turmeric and rosemary extracts in combination may be worthwhile to investigate in the pre-clinical and clinical neoplastic considering there are no negative effects on traditional chemotherapy treatment. Further studies into the pharmacokinetics and mechanisms of action of these extracts should be investigated.
Insights
Turmeric and rosemary extracts show antiproliferative effects in canine cancer cells. Their combination is synergistic with chemotherapy and safe for normal cells, suggesting potential for improved remission rates in canine neoplasia.
Area of Science:
- Veterinary Oncology
- Nutraceuticals in Canine Cancer
- In Vitro Cancer Research
Background:
- Adjunctive nutraceuticals show promise in human cancer but are understudied in canine neoplasia.
- Polyphenols and carotenoids target multiple cancer pathways, potentially interacting with chemotherapy.
- Nutraceuticals in pet food are attractive for canine cancer treatment if remission rates improve.
Purpose of the Study:
- To evaluate the antiproliferative effects of five feed ingredients on canine neoplastic cell lines.
- To assess the interaction of these extracts with toceranib phosphate and doxorubicin hydrochloride.
- To identify potential nutraceuticals for improving canine cancer treatment outcomes.
Main Methods:
- Utilized MTT proliferation assays to screen five feed ingredients.
- Investigated the synergistic or antagonistic effects of extracts with common chemotherapeutic agents.
- Assessed the impact of combination therapies on normal primary canine cells.
Main Results:
- Green tea, turmeric, and rosemary extracts demonstrated significant antiproliferative activity.
- Turmeric extract (TE) was most potent, showing synergy with rosemary extract (RE) between 1-25 μg/mL.
- Combination therapy exhibited additive or synergistic effects with chemotherapy without harming normal cells.
Conclusions:
- Combined turmeric and rosemary extracts warrant further investigation for canine neoplasia.
- These extracts show potential as an adjunct to traditional chemotherapy without adverse interactions.
- Future research should focus on pharmacokinetics and mechanisms of action for these nutraceuticals.
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