Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Clinical Spectrum of Anti-SEZ6L2 Syndrome: A Case Report With Brain Volumetry and Systematic Literature Review.

Neurology(R) neuroimmunology & neuroinflammation·2026

Same author

Septin multimer autoantibodies in severe motor neuropathy mimicking lower motor neuron disease.

Brain : a journal of neurology·2026

Same author

Neuronal autoantibodies associated with poorer neuropsychological and motor outcomes 6 months after stroke: results from the PHYS-STROKE trial.

Frontiers in immunology·2026

Same author

Immune cell profiling reveals expanded stem cell-like memory T cells in anti-GAD65-associated neurological syndromes.

Proceedings of the National Academy of Sciences of the United States of America·2026

Same author

Anti-VGLUT2 autoantibodies associated with post-COVID neurocognitive dysfunction: a case report.

Frontiers in immunology·2026

Same author

Memory Deficits in Cancer Patients With Serum NMDA Receptor Autoantibodies.

Neurology·2025

Search research articles

Related Experiment Video

Updated: Mar 16, 2026

Author Spotlight: Novel Assay for Studying B-Cell Responses in Multiple Sclerosis Research

Author Spotlight: Novel Assay for Studying B-Cell Responses in Multiple Sclerosis Research

Published on: December 1, 2023

Multiparametric serological testing in autoimmune encephalitis using computer-aided immunofluorescence microscopy

Johanna Fraune¹, Stefan Gerlach¹, Kristin Rentzsch¹

¹Institute for Experimental Immunology, Euroimmun AG, Seekamp 31, 23560 Lübeck, Germany.

Autoimmunity Reviews

|August 5, 2016

Summary

This study introduces a new semi-automated system for detecting anti-neuronal autoantibodies, crucial for diagnosing autoimmune encephalitis. Early diagnosis via this method aids prompt treatment and improves patient recovery outcomes.

Keywords:

Anti-neuronal autoantibody Autoimmune encephalitis Computer-aided immunofluorescence microscopy (CAIFM)Recombinant cell-based assay

More Related Videos

Author Spotlight: Unveiling the Pathway Linking Obesity to Autoimmune Inflammation in Multiple Sclerosis

Author Spotlight: Unveiling the Pathway Linking Obesity to Autoimmune Inflammation in Multiple Sclerosis

Published on: February 23, 2024

Hippocampal Neuronal Cultures to Detect and Study New Pathogenic Antibodies Involved in Autoimmune Encephalitis

Hippocampal Neuronal Cultures to Detect and Study New Pathogenic Antibodies Involved in Autoimmune Encephalitis

Published on: June 2, 2022

Related Experiment Videos

Last Updated: Mar 16, 2026

Author Spotlight: Novel Assay for Studying B-Cell Responses in Multiple Sclerosis Research

Author Spotlight: Novel Assay for Studying B-Cell Responses in Multiple Sclerosis Research

Published on: December 1, 2023

Author Spotlight: Unveiling the Pathway Linking Obesity to Autoimmune Inflammation in Multiple Sclerosis

Author Spotlight: Unveiling the Pathway Linking Obesity to Autoimmune Inflammation in Multiple Sclerosis

Published on: February 23, 2024

Hippocampal Neuronal Cultures to Detect and Study New Pathogenic Antibodies Involved in Autoimmune Encephalitis

Hippocampal Neuronal Cultures to Detect and Study New Pathogenic Antibodies Involved in Autoimmune Encephalitis

Published on: June 2, 2022

Area of Science:

Neuroimmunology
Medical Diagnostics

Background:

Autoantibodies targeting neuronal cell surface antigens are key indicators of immunotherapy-responsive autoimmune encephalitis.
Prompt diagnosis is critical to prevent severe neurological damage, as symptoms often overlap, necessitating broad autoantibody screening.

Purpose of the Study:

To evaluate a novel semi-automated function for computer-aided immunofluorescence microscopy in analyzing recombinant cell-based indirect immunofluorescence tests (IIFT).
To enhance the parallel and prompt determination of anti-neuronal autoantibodies for comprehensive differential diagnostics in autoimmune encephalitis.

Main Methods:

Utilized recombinant cell-based immunofluorescence to present membrane-associated surface antigens.
Employed biochip mosaics for multiparametric analysis.
Integrated a semi-automated function into a computer-aided immunofluorescence microscopy platform for IIFT analysis.

Main Results:

The novel system facilitates microscopic analysis of IIFT assays.
It supports laboratory personnel in rapidly issuing diagnostic findings for anti-neuronal autoantibodies.
This aids in timely diagnosis and treatment initiation for autoimmune encephalitis.

Conclusions:

The developed semi-automated system improves the efficiency and speed of diagnosing autoimmune encephalitis by enabling rapid detection of anti-neuronal autoantibodies.
This technological advancement is vital for comprehensive differential diagnostics and timely therapeutic intervention, potentially leading to full patient recovery.