Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Mouse embryos cultured in amniotic fluid.

E E Oettlé1, K Wiswedel

  • 1Department of Obstetrics and Gynaecology, University of Cape Town.

South African Medical Journal = Suid-Afrikaanse Tydskrif Vir Geneeskunde
|July 15, 1989
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Prospective study of hormonal and semen profiles in marathon runners.

Fertility and sterility·1995
Same author

Assessment of acrosomal integrity of vervet monkey spermatozoa after cryopreservation.

Journal of medical primatology·1994
Same author

Cryopreservation of vervet monkey semen and recovery of progressively motile spermatozoa.

Journal of medical primatology·1993
Same author

Sperm morphology and fertility in the dog.

Journal of reproduction and fertility. Supplement·1993
Same author

Ultrastructural parameters of fertile cryopreserved human sperm.

Archives of andrology·1992
Same author

The embryo freezing programme at Groote Schuur Hospital. First results.

South African medical journal = Suid-Afrikaanse tydskrif vir geneeskunde·1990

Mouse embryos cultured in amniotic fluid showed improved blastocyst development compared to Earle's medium. This suggests that amniotic fluid is a viable medium for mouse embryo culture.

Area of Science:

  • Reproductive biology
  • Developmental biology
  • In vitro fertilization

Background:

  • Optimizing in vitro culture media is crucial for successful embryo development.
  • Amniotic fluid's composition and potential as a culture medium for embryos are not fully understood.

Purpose of the Study:

  • To evaluate the efficacy of human amniotic fluid as a culture medium for mouse embryos.
  • To compare the developmental potential of mouse embryos cultured in amniotic fluid versus a standard Earle's medium.

Main Methods:

  • One thousand mouse embryos were cultured for 72 hours in either amniotic fluid or Earle's medium.
  • Embryo development was assessed based on stages: degenerate, morula, and blastocyst.
  • Key culture parameters (osmolarity, pH, CO2, HCO3-) were monitored.

Related Experiment Videos

Main Results:

  • A significantly higher rate of blastulation to fully expanded blastocysts was observed in embryos cultured with amniotic fluid.
  • Amniotic fluid exhibited variability in composition, pH, and osmolarity.
  • Earle's medium demonstrated consistent composition across trials.

Conclusions:

  • Human amniotic fluid collected at 16 weeks gestation from normal pregnancies serves as an effective culture medium for mouse embryos.
  • Amniotic fluid supports superior blastocyst development compared to Earle's medium.