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Updated: Mar 16, 2026

Simple In-House Ultra-High Performance Capillary Column Manufacturing with the FlashPack Approach
Published on: December 4, 2021
Implementation of high slurry concentration and sonication to pack high-efficiency, meter-long capillary ultrahigh
Justin M Godinho1, Arved E Reising2, Ulrich Tallarek2
1Department of Chemistry, The University of North Carolina at Chapel Hill, Chapel Hill, NC, 27599-3290, United States.
Abstract:
Slurry packing capillary columns for ultrahigh pressure liquid chromatography is complicated by many interdependent experimental variables. Previous results have suggested that combination of high slurry concentration and sonication during packing would create homogeneous bed microstructures and yield highly efficient capillary columns. Herein, the effect of sonication while packing very high slurry concentrations is presented. A series of six, 1m×75μm internal diameter columns were packed with 200mg/mL slurries of 2.02μm bridged-ethyl hybrid silica particles. Three of the columns underwent sonication during packing and yielded highly efficient separations with reduced plate heights as low as 1.05.
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