The Development of Fluorescence Intensity Standards.
A K Gaigalas1, L Li1, O Henderson2
1National Institute of Standards and Technology, Gaithersburg, MD 20899-8312.
Summary
Developing primary fluorescence intensity standards is feasible, addressing the need for comparable measurements across labs. This will advance analytical techniques by enabling reliable fluorescence intensity standard reference materials.
Area of Science:
- Analytical Chemistry
- Spectroscopy
Background:
- Fluorescence is a growing analytical technique, but its advancement is hindered by inconsistent intensity measurements across different laboratories.
- The lack of standardized fluorescence intensity measurements limits the reliability and comparability of results in scientific assays.
Purpose of the Study:
- To address the need for comparable fluorescence intensity measurements by developing primary fluorescence intensity standard (FIS) reference materials.
- To facilitate the broader adoption and accuracy of fluorescence-based analytical techniques.
Main Methods:
- The National Institute of Standards and Technology (NIST) recognizes the necessity of creating primary FIS reference materials.
- Collaboration between federal laboratories, manufacturers, and end-users is identified as crucial for FIS development.
Main Results:
- The development and implementation of FIS are acknowledged to present challenges.
- The article outlines the feasibility of developing these essential fluorescence intensity standards.
Conclusions:
- The development of primary fluorescence intensity standards is achievable and necessary.
- Establishing FIS will overcome current limitations in fluorescence intensity measurements, promoting greater accuracy and inter-laboratory consistency.
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