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Updated: Mar 16, 2026
13:08
Measurement of Factor V Activity in Human Plasma Using a Microplate Coagulation Assay
Published on: September 9, 2012
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Correlation between factor VII and PAI-1 genetic variants and recurrent miscarriage
Magdalena Barlik1, Agnieszka Seremak-Mrozikiewicz, Krzysztof Drews
1Department of Perinatology and Women's Diseases, Poznan University of Medical Sciences, Poland Laboratory of Molecular Biology in Division of Perinatology and Women's Diseases, Poznan University of Medical Sciences, Poznan, Poland. magda.barlik@op.pl.
Ginekologia Polska
|August 10, 2016
Summary
Genetic polymorphisms in factor VII and PAI-1 genes may influence inherited thrombophilia and recurrent miscarriage. Certain factor VII gene variants (Gln353 and -122C) may offer a protective effect against pregnancy loss.
Area of Science:
- Genetics
- Reproductive Medicine
- Thrombophilia
Background:
- Inherited thrombophilia, linked to specific gene polymorphisms, is a potential cause of recurrent pregnancy loss.
- Cardiology studies suggest these polymorphisms contribute to thrombotic events.
Purpose of the Study:
- To investigate the association between factor VII gene polymorphisms (Arg353Gln, -122T > C) and PAI-1 gene polymorphism (-675 4G/5G) and the etiology of recurrent miscarriage.
- To evaluate the potential role of these genetic variations in women experiencing repeated pregnancy losses.
Main Methods:
- A case-control study involving 152 women with a history of recurrent miscarriage (≥ 2 consecutive losses) and 180 healthy controls.
- Genetic analysis using Polymerase Chain Reaction with Restriction Fragment Length Polymorphism (PCR/RFLP) to identify specific gene variants.
Main Results:
- A lower frequency of Arg353/Gln353 and Gln353 alleles was observed in women with recurrent miscarriages compared to controls.
- The -122TT genotype and -122T allele were more frequent in women with ≥ 3 miscarriages.
- No significant association was found for the PAI-1 gene -675 4G/5G polymorphism.
Conclusions:
- The findings suggest that the Gln353 allele of the factor VII gene and the -122C allele may have a protective role against recurrent miscarriage.
- Further research is warranted to elucidate the precise mechanisms by which these polymorphisms influence pregnancy outcomes.
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