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Temporal Sensing Platform Based on Bipolar Electrode for the Ultrasensitive Detection of Cancer Cells
Hai-Wei Shi1, Wei Zhao1, Zhen Liu1
1State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University , Nanjing 210023, China.
Analytical Chemistry
|August 11, 2016
Summary
This study introduces a novel bipolar electrode (BPE) sensing platform for detecting cancer cells using anodic dissolution and electrochemiluminescence (ECL). The innovative method achieves ultra-low detection limits for cancer cell quantification.
Area of Science:
- Electrochemistry
- Biosensing
- Nanotechnology
Background:
- Cancer cell detection remains a critical challenge in diagnostics.
- Existing methods often lack sensitivity and require large sample volumes.
- Bipolar electrode (BPE) platforms offer potential for sensitive electrochemical detection.
Purpose of the Study:
- To develop a BPE sensing platform for ultrasensitive temporal detection of cancer cells.
- To combine anodic dissolution and electrochemiluminescence (ECL) for enhanced sensitivity.
- To investigate the capture efficiencies of antibodies and aptamers for cancer cell monitoring.
Main Methods:
- Utilized a BPE platform with gold nanoparticles (Au NPs) on the anode for ECL catalysis and silver (Ag) deposition.
- Employed anodic dissolution of Ag, correlating dissolution time with conductivity changes.
- Monitored ECL emission recovery to quantify cancer cells based on conductivity variations.
Main Results:
- Achieved an ultra-low detection limit of 5 cells/cm(2) for cancer cells.
- Demonstrated that ECL emission recovery time accurately reflects anodic dissolution extent.
- Successfully quantified cancer cells by monitoring conductivity changes via ECL recovery.
Conclusions:
- The combined anodic dissolution and ECL strategy significantly enhances BPE device sensitivity.
- This approach enables ultrasensitive monitoring of cancer cells.
- The platform is applicable for evaluating antibody and aptamer capture efficiencies for specific cancer cell lines (MCF-7, A549).
