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Long-term Behavioral and Reproductive Consequences of Embryonic Exposure to Low-dose Toxicants
Published on: March 6, 2018
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A novel approach to assessing bisphenol-A hazards using an in vitro model system
Md Saidur Rahman1, Woo-Sung Kwon1, Sung-Jae Yoon1
1Department of Animal Science and Technology, Chung-Ang University, Anseong, Gyeonggi-Do, 456-756, Republic of Korea.
BMC Genomics
|August 11, 2016
Summary
Bisphenol-A (BPA) exposure negatively impacts sperm function and viability by activating specific kinase pathways. BPA also alters sperm protein profiles, potentially contributing to disease development, including infertility and cancer.
Area of Science:
- Toxicology
- Reproductive Biology
- Proteomics
Background:
- Bisphenol-A (BPA) is a widespread xenoestrogen with poorly understood mechanisms of action.
- Standardized methods for assessing BPA's health hazards are lacking.
- Mice spermatozoa serve as an in vitro model to investigate BPA's effects and mechanisms.
Purpose of the Study:
- To investigate the effects of BPA exposure on spermatozoa and elucidate the underlying mechanisms.
- To analyze the sperm proteome changes in response to BPA exposure.
- To explore the potential link between BPA-induced sperm alterations and disease pathogenesis.
Main Methods:
- Exposure of mice spermatozoa to varying concentrations of BPA (0.0001–100 μM) for 6 hours.
- Assessment of sperm motility, viability, mitochondrial function, and intracellular ATP levels.
- Proteomic analysis to identify differentially expressed proteins in BPA-treated spermatozoa.
Main Results:
- High BPA concentrations impaired sperm motility, viability, mitochondrial function, and ATP levels.
- BPA activated mitogen-activated protein kinase, phosphatidylinositol 3-kinase, and protein kinase-A pathways.
- Differential expression of 24 proteins was observed, linked to protein degradation and phosphorylation, potentially involved in cancer and infertility.
Conclusions:
- BPA adversely affects sperm function via kinase pathway activation.
- BPA-induced proteomic changes in sperm may contribute to observed functional deficits and disease pathogenesis.
- This approach may be valuable for assessing health hazards of other toxic agents.
Keywords:
Bioinformatics databasesBisphenol-ADiseasesHealth hazardsIn-vitroProteomicsSignaling pathwaysSpermatozoa
