A Venn diagram model that allows triple-label immunophenotypic analysis of cells based upon double-label measurements

Using Venn diagrams derived from set theory, a mathematic model is presented that allows triple-label immunophenotypic analysis of cells based upon double-label measurements. Practical applications of this method are illustrated and discussed. Using this model, triple-label data concerning Leu-8 and Leu-9 (CD7) antigen co-expression by Leu-4+ (CD3+) leukocytes were derived from double-label measurements obtained with a single laser flow cytometer. Mean values were as follows: Leu-4+8+9+ (62%), Leu-4+8-9+ (36%), Leu-4+8+9- (0%), and Leu-4+8-9- (0%). Examples are given of additional models based upon similar principles.