Related Experiment Video
Updated: Mar 16, 2026
Bridging the Bio-Electronic Interface with Biofabrication
Published on: June 6, 2012
Data on biochemical fluxes generated from biofabricated enzyme complexes assembled through engineered tags and
Narendranath Bhokisham1, Haig Pakhchanian2, David Quan3
1Biological Sciences Graduate Program - College of Computer, Mathematical and Natural Sciences, University of Maryland, 4066 Campus Drive, College Park, MD 20742, United States; Institute of Bioscience and Biotechnology Research, University of Maryland, College Park, 5115 Plant Science and Landscape Architecture Building, College Park, MD 20742, United States.
Abstract:
Data presented is related to an article titled "Modular construction of multi-subunit protein complexes using engineered tags and microbial transglutaminase" (Bhokisham et al., 2016) [1]. In this article, we have presented western blot and flux data associated with assembly of Pfs-LuxS enzyme complexes on beads using uni-tagged and bi-tagged LuxS enzymes. We have also presented biochemical flux following changes in enzyme stoichiometries. We covalently coupled a Pfs-LuxS complex with Protein G, an antibody binding non-enzyme component and directed these complexes to the surfaces of bacterial cells via anti-Escherichia coli antibodies. Fluorescence microscopy images represented the altered behavior of bacterial cells in response to the autoinducer-2 that is synthesized by the Protein G-enzyme complexes.
