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An endpoint enzymatic assay for fructose 2,6-bisphosphate performed in 96-well plates
P Bruni1, V Vasta, M Farnararo
1Department of Biochemistry, University of Florence, Firenze, Italy.
Analytical Biochemistry
|May 1, 1989
Abstract:
An endpoint enzymatic assay for fructose 2,6-bisphosphate based on the ability of the compound to stimulate pyrophosphate 6-phosphofructo-1-kinase and performed in a 96-well plate is reported here. The method presents a low detection limit and a high sensitivity that could be further improved; moreover, the use of 96-well plates greatly increases the number of samples that can be simultaneously assayed.