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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Karyotyping

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Updated: Mar 16, 2026

A Fast Air-dry Dropping Chromosome Preparation Method Suitable for FISH in Plants

A Fast Air-dry Dropping Chromosome Preparation Method Suitable for FISH in Plants

Published on: December 16, 2015

Flow Sorting Plant Chromosomes.

Jan Vrána¹, Petr Cápal², Jarmila Číhalíková²

¹Centre of the Region Haná for Biotechnological and Agricultural Research, Institute of Experimental Botany, Šlechtitelů 31, 783 71, Olomouc, Czech Republic. vrana@ueb.cas.cz.

Methods in Molecular Biology (Clifton, N.J.)

|August 12, 2016

Summary

This summary is machine-generated.

This study details a method for isolating intact plant chromosomes using flow cytometry. This technique provides pure chromosome fractions essential for advanced plant genomics research.

Keywords:

Cell cycle synchronization Chromosome isolation Cytogenetic stocks FISH FISHIS Flow cytometry and sorting Metaphase accumulation Plants

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Last Updated: Mar 16, 2026

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Published on: February 18, 2010

Isolation and Transcriptome Analysis of Plant Cell Types

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Published on: April 7, 2023

Area of Science:

Plant genomics
Cytogenetics
Molecular biology

Background:

Nuclear genomes in plants are complex and challenging to map and sequence.
Isolating single chromosomes is crucial for various plant research fields.

Purpose of the Study:

To describe a detailed protocol for preparing liquid suspensions of intact mitotic metaphase chromosomes.
To enable flow cytometric analysis and sorting of these chromosomes.

Main Methods:

Preparation of intact mitotic metaphase chromosomes from plant tissues.
Flow cytometric analysis for chromosome characterization.
Flow sorting for isolation of pure chromosome fractions.

Main Results:

Successful isolation of intact mitotic metaphase chromosomes in liquid suspension.
Demonstration of flow cytometric analysis and sorting capabilities for plant chromosomes.

Conclusions:

Flow cytometry is the premier method for obtaining pure chromosome fractions from plants.
This protocol facilitates downstream applications like physical mapping and sequencing.