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Generation of Dynamical Environmental Conditions using a High-Throughput Microfluidic Device

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Published on: April 17, 2021

A drug-compatible and temperature-controlled microfluidic device for live-cell imaging.

Tong Chen¹, Blanca Gomez-Escoda², Javier Munoz-Garcia¹

¹SyntheCell team, Institute of Genetics and Development, CNRS UMR 6290, 2 avenue du Pr. Léon Bernard, 35043 Rennes, France.

|August 12, 2016

Summary

Researchers developed new polydimethylsiloxane (PDMS)-free microdevices for live-cell imaging. These advanced platforms enable precise control over cellular microenvironments, overcoming limitations of previous technologies for enhanced biological studies.

Keywords:

cell biology control of cellular environment live-cell imaging microfluidics microscopy

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Last Updated: Mar 16, 2026

Generation of Dynamical Environmental Conditions using a High-Throughput Microfluidic Device

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Published on: April 17, 2021

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A Flexible Chamber for Time-Lapse Live-Cell Imaging with Stimulated Raman Scattering Microscopy

A Flexible Chamber for Time-Lapse Live-Cell Imaging with Stimulated Raman Scattering Microscopy

Published on: August 31, 2022

Area of Science:

Cell Biology
Bioengineering
Microfluidics

Background:

Live-cell imaging is crucial for studying biological processes.
Current microfluidic devices often use polydimethylsiloxane (PDMS), which interferes with small molecules.
This interference affects the accuracy of modulating cellular microenvironments during experiments.

Purpose of the Study:

To develop novel PDMS-free microdevices for live-cell imaging.
To overcome the limitations of molecule absorption by conventional microfluidic materials.
To enable precise control over cellular microenvironments, including temperature and medium composition.

Main Methods:

Fabrication of PDMS-free microdevices for live-cell imaging.
Integration of temperature control modules for precise sample temperature regulation (above and below ambient).
Demonstration of dynamic changes in medium composition and temperature during microscopy observation.

Main Results:

The new microdevices exhibit no interference with small molecules, ensuring accurate experimental conditions.
Precise temperature control and rapid temperature shifts are achievable within the microdevices.
Dynamic modulation of the cellular environment, including medium composition and temperature, is demonstrated during live-cell imaging.

Conclusions:

The developed PDMS-free microdevices offer a versatile solution for live-cell imaging.
These platforms enhance the reliability of experiments by preventing unintended molecule absorption.
They facilitate dynamic regulation of the cellular environment for advanced biological investigations.