Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

When Mr. Fap Meets the Gals.

Santosh K Ghosh1, Aaron Weinberg1

  • 1Biological Sciences, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106, USA.

Cell Host & Microbe
|August 12, 2016
PubMed
Summary

Fusobacteria are overrepresented in colorectal tumors. A novel mechanism shows these bacteria use a specific sugar (Gal-GalNAc) on cancer cells, recognized by the Fap2 lectin, to home in on tumors.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Attentive fidelity and the coordination of attentive and conceptual processes in learning from mathematics videos lessons.

Acta psychologica·2026
Same author

Beta-defensin index: A functional biomarker for oral cancer detection.

Cell reports. Medicine·2024
Same author

Impact of Molnupiravir Treatment on Patient-Reported COVID-19 Symptoms in the Phase 3 MOVe-OUT Trial: A Randomized, Placebo-Controlled Trial.

Clinical infectious diseases : an official publication of the Infectious Diseases Society of America·2023
Same author

Human β-Defensin-3 is Associated With Platelet-Derived Extracellular Vesicles and is a Potential Contributor to Endothelial Dysfunction.

Frontiers in molecular biosciences·2022
Same author

HBD-2 binds SARS-CoV-2 RBD and blocks viral entry: Strategy to combat COVID-19.

iScience·2022
Same author

Ramping Up Antimicrobial Peptides Against Severe Acute Respiratory Syndrome Coronavirus-2.

Frontiers in molecular biosciences·2021

Area of Science:

  • Microbiology
  • Oncology
  • Molecular Biology

Background:

  • Fusobacteria are frequently detected in the colorectal tumor microenvironment.
  • Their specific role and localization mechanisms within tumors remain incompletely understood.

Purpose of the Study:

  • To elucidate the mechanism by which fusobacteria specifically localize to colorectal tumors.
  • To identify bacterial factors and host molecules involved in this tumor-homing process.

Main Methods:

  • Investigated the interaction between fusobacteria and colorectal cancer cells.
  • Utilized lectin binding assays and genetic manipulation of fusobacteria.
  • Analyzed host cell surface carbohydrate expression.

Main Results:

  • Fusobacteria employ a lectin, Fap2, to bind to a specific host polysaccharide, Gal-GalNAc.
  • This Gal-GalNAc is overexpressed on colorectal cancer cells, mediating fusobacterial tumor homing.
  • Fap2-mediated binding is crucial for fusobacterial colonization of the tumor microenvironment.

Conclusions:

  • Fusobacteria possess a specific homing mechanism to target colorectal tumors.
  • The Fap2 lectin-Gal-GalNAc interaction is a key determinant of fusobacterial localization in colorectal cancer.

Related Experiment Videos