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Identification of Coding and Non-coding RNA Classes Expressed in Swine Whole Blood
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Globin mRNA reduction for whole-blood transcriptome sequencing.

Kaarel Krjutškov1,2,3, Mariann Koel2,4, Anne Mari Roost2

  • 1Department of Biosciences and Nutrition, Karolinska Institutet, Huddinge, Sweden.

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|August 13, 2016
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Summary
This summary is machine-generated.

Researchers developed GlobinLock (GL), a novel assay to reduce high globin mRNA in whole-blood RNA sequencing. This method preserves RNA quality, saves time and cost, and works across multiple species.

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Area of Science:

  • Molecular Biology
  • Genomics
  • Biotechnology

Background:

  • Whole-blood RNA sequencing is valuable for biomarker discovery.
  • High globin mRNA in erythrocytes complicates whole-blood and buffy coat RNA analysis.

Purpose of the Study:

  • To introduce a novel, robust, and simple tool for reducing globin mRNA in whole-blood RNA samples.
  • To preserve RNA quality, save time, and reduce costs associated with RNA sequencing.

Main Methods:

  • Development of a novel globin mRNA locking assay (GlobinLock, GL).
  • GL utilizes gmRNA-specific oligonucleotides in RNA denaturation buffer.
  • Assay adds only ten minutes to the cDNA synthesis procedure.

Main Results:

  • GlobinLock effectively reduces globin mRNA content in whole-blood RNA.
  • The assay preserves RNA quality for downstream analyses.
  • GL is effective for human, mouse, rat, cow, dog, and zebrafish samples.

Conclusions:

  • GlobinLock is a simple and effective method for reducing globin mRNA in various species.
  • This assay facilitates more efficient and cost-effective whole-blood RNA sequencing.
  • GL enhances the utility of whole-blood RNA for biomarker identification and tracking.