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Updated: Mar 16, 2026
Detection of Neu1 Sialidase Activity in Regulating TOLL-like Receptor Activation
Published on: September 7, 2010
An optimised assay for quantitative, high-throughput analysis of polysialyltransferase activity
Sara M Elkashef1, Mark Sutherland, Laurence H Patterson
1Institute of Cancer Therapeutics, Faculty of Life Sciences, University of Bradford, Bradford BD7 1DP, UK. r.a.falconer1@bradford.ac.uk.
We developed a sensitive, high-throughput assay to measure human polysialyltransferase activity. This tool aids in understanding polysialic acid
Area of Science:
- Biochemistry
- Glycobiology
- Enzymology
Background:
- Polysialyltransferases are key glycosyltransferases synthesizing polysialic acid.
- Polysialic acid is implicated in cancer progression and other diseases.
Purpose of the Study:
- To optimize and validate a sensitive, cell-free, high-throughput HPLC-based assay.
- To assess human polysialyltransferase activity.
Main Methods:
- Improved chemical synthesis and purification of a fluorescently-labelled DMB-DP3 acceptor.
- Development and validation of a high-throughput HPLC assay.
Main Results:
- A highly sensitive cell-free assay for polysialyltransferase activity was established.
- The assay utilizes an optimized fluorescently-labelled DMB-DP3 acceptor.
Conclusions:
- The developed assay provides a robust method for measuring human polysialyltransferase activity.
- This assay facilitates research into the role of polysialic acid in disease.
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