Related Experiment Video
Updated: Mar 16, 2026

Enzymatic Synthesis of Epoxidized Metabolites of Docosahexaenoic, Eicosapentaenoic, and Arachidonic Acids
Published on: June 28, 2019
Synthesis and characterization of [1-(13)C]-and d8-arachidonic acid
U H Do1, M G Sundaram1, S Ramachandran1
1Applied Science Division, Milton Roy Company, 16801, State College, Pennsylvania.
Abstract:
Methyld 8- and [1-(13)C] 5,8,11,14-eicosatetraenoate (arachidonate) were prepared from a common synthetic precursor, 4,7,10,13-nonadecatetrayn-1-ol. The purified products were characterized by gas chromatography-mass spectrometry. Mass spectra oft-butyldimethylsilyl esters ofd 8-and [1-(13)C]-arachidonic acid showed a most intense [M-57](+) peak at high mass. The isotopic purity of methyl [1-(13)C] arachidonate was 99% and that of methyld 8-arachidonate was 56%. Whend 8-arachidonic acid was prepared by direct deuteration of 5,8,11,14-eicosatetraynoic acid, the isotopic purity of the sample was 86%.
More Related Videos
11:14Fatty Acid 13C Isotopologue Profiling Provides Insight into Trophic Carbon Transfer and Lipid Metabolism of Invertebrate Consumers
Published on: April 17, 2018
14:25Synthesis of a Deuterated Standard for the Quantification of 2-Arachidonoylglycerol in Caenorhabditis elegans
Published on: September 21, 2019
Related Concept Videos
NMR and Mass Spectroscopy of Carboxylic Acids
While α protons of carboxylic acids absorb at 2–2.5 ppm, β protons absorb further upfield.
Carboxylic acids are easily identified by dissolving them in deuterium oxide, which results in a rapid exchange of the acidic protons with deuterium. This leads to the...
Carboxylic Acids to Methylesters: Alkylation using Diazomethane
Carbon-13 (¹³C) NMR: Overview
Synthesis of Phosphatidylcholine in the ER Membrane
The major components of all eukaryotic cell...
Carboxylic Acids to Esters: Acid-Catalyzed (Fischer) Esterification Mechanism
Alkylation of β-Diester Enolates: Malonic Ester Synthesis