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Related Experiment Videos

Membrane structure in isolated and intact myelins.

H Inouye1, J Karthigasan, D A Kirschner

  • 1Department of Neurology Research, Children's Hospital, Boston, Massachusetts.

Biophysical Journal
|July 1, 1989
PubMed
Summary

Isolated myelin structure is largely similar to intact myelin, despite differences in membrane interactions. Electron density profiles show no major structural changes that explain these interaction variations in myelin research.

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Area of Science:

  • Neuroscience
  • Biochemistry
  • Structural Biology

Background:

  • Myelin structure and composition are typically studied using isolated membranes.
  • Previous studies indicated differences in membrane interactions between intact and isolated myelin.
  • The current study investigates if altered membrane structure in isolated myelin explains these interaction differences.

Purpose of the Study:

  • To compare the membrane structure of isolated myelin with that of intact myelin.
  • To determine if structural changes in isolated myelin account for observed differences in intermembrane interactions.

Main Methods:

  • Development of nonlinear least squares procedures for analyzing x-ray scattering data from isolated myelin.
  • Calculation of electron density profiles for isolated myelin.

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  • Comparison of electron density profiles with those of intact myelin across varying pH and ionic strength.

    • Main Results:

    • The behavior of extracellular and cytoplasmic spaces in isolated myelin largely paralleled that of intact myelin with changes in pH and ionic strength.
    • Exceptions noted: isolated CNS myelin resisted swelling, and isolated PNS myelin showed indefinite swelling in hypotonic saline.
    • Electron density profiles (to 30 Å resolution) of isolated myelin revealed no significant structural alterations compared to intact myelin.

    Conclusions:

    • Major structural changes in isolated myelin do not explain the previously observed differences in intermembrane interactions.
    • The biochemical composition and topology of isolated myelin are broadly similar to intact myelin.
    • Further research is needed to understand the basis of altered intermembrane interactions in isolated myelin preparations.