Fluorescence-linked Antigen Quantification (FLAQ) Assay for Fast Quantification of HIV-1 p24^Gag

The fluorescence-linked antigen quantification (FLAQ) assay allows a fast quantification of HIV-1 p24^Gag antigen. Viral supernatant are lysed and incubated with polystyrene microspheres coated with polyclonal antibodies against HIV-1 p24^Gag and detector antibodies conjugated to fluorochromes (Figure 1). After washes, the fluorescence of microspheres is measured by flow cytometry and reflects the abundance of the antigen in the lysate. The speed, simplicity, and wide dynamic range of the FLAQ assay are optimum for many applications performed in HIV-1 research laboratories.