Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

The histone demethylase UTX regulates the lineage-specific epigenetic program of invariant natural killer T cells.

Nature immunology·2016

Same author

Corrigendum: LSD1 is essential for oocyte meiotic progression by regulating CDC25B expression in mice.

Nature communications·2016

Same author

LSD1 is essential for oocyte meiotic progression by regulating CDC25B expression in mice.

Nature communications·2015

Same author

The LSD1 Family of Histone Demethylases and the Pumilio Posttranscriptional Repressor Function in a Complex Regulatory Feedback Loop.

Molecular and cellular biology·2015

Same author

Scl binds to primed enhancers in mesoderm to regulate hematopoietic and cardiac fate divergence.

The EMBO journal·2015

Same author

Corepressor Rcor1 is essential for murine erythropoiesis.

Blood·2014

Search research articles

Related Experiment Video

Updated: Mar 16, 2026

Colony Forming Cell CFC Assay for Human Hematopoietic Cells

Colony Forming Cell CFC Assay for Human Hematopoietic Cells

Published on: December 18, 2010

LT-HSC Methylcellulose Assay.

Marc A Kerenyi¹

¹Division of Hematology/Oncology, Boston Children's Hospital and Dana Farber Cancer Institute, Harvard Medical School, Boston, USA.

|August 16, 2016

Summary

This summary is machine-generated.

This study introduces a new methylcellulose colony-forming assay to directly assess long-term repopulating hematopoietic stem cell (LT-HSC) differentiation capacity. This in vitro method complements existing techniques for evaluating stem cell function.

More Related Videos

Isolation of Murine Embryonic Hemogenic Endothelial Cells

Isolation of Murine Embryonic Hemogenic Endothelial Cells

Published on: June 17, 2016

A Quantitative Assay for Insulin-expressing Colony-forming Progenitors

A Quantitative Assay for Insulin-expressing Colony-forming Progenitors

Published on: November 28, 2011

Related Experiment Videos

Last Updated: Mar 16, 2026

Colony Forming Cell CFC Assay for Human Hematopoietic Cells

Colony Forming Cell CFC Assay for Human Hematopoietic Cells

Published on: December 18, 2010

Isolation of Murine Embryonic Hemogenic Endothelial Cells

Isolation of Murine Embryonic Hemogenic Endothelial Cells

Published on: June 17, 2016

A Quantitative Assay for Insulin-expressing Colony-forming Progenitors

A Quantitative Assay for Insulin-expressing Colony-forming Progenitors

Published on: November 28, 2011

Area of Science:

Hematology
Stem Cell Biology
Cell Differentiation

Background:

Hematopoietic stem cells (HSCs) are crucial for blood cell production, with long-term repopulating HSCs (LT-HSCs) maintaining the stem cell pool through asymmetric division.
Assessing LT-HSC function typically involves multi-color flow cytometry and competitive bone marrow transplantation, which have limitations in distinguishing self-renewal from differentiation defects.

Purpose of the Study:

To introduce a novel, fast, in vitro methylcellulose colony-forming assay for directly evaluating LT-HSC differentiation capacity.
To provide a complementary method to existing techniques for a more precise assessment of stem and progenitor cell differentiation.

Main Methods:

Development and application of an LT-HSC methylcellulose colony-forming assay.
Utilizing the assay as a complementary in vitro tool to assess differentiation capacity.

Main Results:

The methylcellulose colony-forming assay provides a direct measure of LT-HSC differentiation capacity.
This assay serves as a valuable tool to differentiate between defects in LT-HSC differentiation and progenitor cell differentiation.

Conclusions:

The described methylcellulose colony-forming assay is a rapid and effective complementary method for assessing LT-HSC differentiation.
This technique enhances the ability to diagnose specific defects within the hematopoietic stem and progenitor cell compartments.