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Updated: Aug 17, 2026
06:16
High Throughput Screening of Fungal Endoglucanase Activity in Escherichia coli
Published on: August 13, 2011
Simple enzymatic screening assay for ethylene glycol (ethane-1,2-diol) in serum
Abstract:
We present a novel method for the analysis of ethylene glycol (ethane-1,2-diol) in serum. This method employs the ability of glycerol dehydrogenase (EC 1.1.1.6) to oxidize ethylene glycol. Two measurements, five and 30 min after addition of enzyme, are used to circumvent interferences from endogenous glycerol and from propylene glycol (propane-1,2-diol). Ethanol does not interfere in the assay. The method requires commonly available equipment only, making it suitable for all emergency laboratories.
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