[The Test Performance and the Differences between Sites of Blood Collection Using the Point of Care Testing-Enabled...

The point-of-care testing (POCT) has been widely performed as decentralized laboratory testing at the site of patient care. Antsense ROSE (ROSE), a POCT device for measurement of blood glucose, is handheld and time-saving and reduces risks of infection because disposable tips are used. In this study, we evaluated the testing performance of ROSE. This study was approved by the ethics committee of Nagasaki University Graduate School of Biomedical Sciences. Clinical blood samples were used for evaluating the reproducibility of testing, the calibration stability, the linearity of dilution, and the effects of dissolved oxygen partial pressure hematocrit, coexisting materials, and anticoagulant agents on the results of ROSE. Blood glucose values during 75g oral glucose tolerance testing (OGTT) were measured for analysis of the differences between sites of blood collection. Comparing with the automatic analyzer based on the hexokinase assay, the accuracy of blood glucose values measured by ROSE was evaluated. ROSE showed good performances in reproducibility, calibration stability, and dilution linearity. The values measured by ROSE were stable to oxygen partial pressure, hematocrit, coexisting materials, and anticoagulant agents and were correlated with those by the automatic analyzer. The values in capillary blood obtained from the ear or the finger elevated as approximately 30-70% as those in venous blood, at 0.5 and 1 hour after OGTT. The inhibitory effect by sodium fluoride (NaF) was 5-25% decrease after 24 hours. In conclusion, ROSE provides the blood glucose values accurately and is a rapid and useful device as a POCT.