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A novel Direct MIC-gradient Strip Method to screen for antibiotic-resistant faecal Enterobacteriaceae
Karianne Wiger Gammelsrud1, Per Kristian Knudsen2, E Arne Høiby3
1Department of Microbiology, Division of Laboratory Medicine, Oslo University Hospital, PB 4956 Nydalen, NO-0424 Oslo, Norway; Faculty of Medicine, University of Oslo, PB 1171 Blindern, NO-0318 Oslo, Norway.
Journal of Microbiological Methods
|August 17, 2016
Summary
A new method uses MIC-gradient strips on agar plates to detect resistant bacteria in faeces. This approach accurately identifies antimicrobial susceptibility patterns and detects rare resistant bacterial strains.
Area of Science:
- Microbiology
- Antimicrobial Resistance
- Clinical Diagnostics
Background:
- Enterobacteriaceae are a significant source of antimicrobial resistance.
- Accurate detection of resistant bacterial populations is crucial for effective treatment.
- Existing methods may not efficiently detect low-frequency resistant subpopulations.
Purpose of the Study:
- To develop and validate a novel method for detecting resistant faecal Enterobacteriaceae.
- To assess the method's ability to determine antimicrobial susceptibility patterns.
- To evaluate the sensitivity in detecting low-prevalence resistant bacterial subpopulations.
Main Methods:
- A novel technique involving the direct application of MIC-gradient strips onto faecal-inoculated agar plates.
- Culturing and analysis of bacterial growth on the agar plates to determine Minimum Inhibitory Concentrations (MICs).
- Evaluation of the method's sensitivity for detecting resistant subpopulations at a frequency of 1 in 100,000 (1/10^5).
Main Results:
- The novel method successfully provided the antimicrobial susceptibility pattern (MICs) of the dominant faecal Enterobacteriaceae population.
- The technique demonstrated high sensitivity, capable of detecting resistant subpopulations present at a frequency of 1/10^5.
- Direct application of MIC-gradient strips offers a streamlined approach compared to traditional methods.
Conclusions:
- A novel, sensitive, and direct method for detecting resistant faecal Enterobacteriaceae has been established.
- This technique allows for the characterization of both dominant and minor resistant bacterial populations.
- The method holds promise for improved diagnostics in antimicrobial resistance surveillance and clinical settings.
