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Updated: Mar 16, 2026

Nucleoside Triphosphates - From Synthesis to Biochemical Characterization
Published on: April 3, 2014
NMR studies on 4-thio-5-furan-modified and 4-thio-5-thiophene-modified nucleosides
Xiao-Hui Zhang1, Yao-Zhong Xu2
1College of Environment and Chemical Engineering, Dalian University, Dalian, 116622, China. zhangxiaohui@dlu.edu.cn.
Abstract:
Systematic NMR characterization of 4-thio-5-furan-pyrimidine nucleosides or 4-thio-5-thiophene-pyrimidine nucleosides (ribonucleosides and 2'-deoxynucleosides) was performed. All proton and carbon signals of 4-thio-5-thiophene-ribouridine and related analogues were unambiguously assigned. The orientations of the base (4-thiouridine or its deoxy analogue) relative to the ring (furan or thiophene) are explored by a NMR approach and further supported by X-ray crystallographic studies. The procedures presented here would be applicable to other modified nucleosides and nucleotides. Copyright © 2016 John Wiley & Sons, Ltd.
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