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Colorimetric Sensor Array for Thiols Discrimination Based on Urease-Metal Ion Pairs.

Chunyang Lei¹, Huang Dai¹, Yingchun Fu¹

¹College of Biosystems Engineering and Food Science, Zhejiang University , Hangzhou, Zhejiang 310058, China.

Analytical Chemistry

|August 18, 2016

Summary

This study introduces a novel colorimetric sensor array for distinguishing thiols. The array utilizes urease-metal ion interactions to create unique color patterns, enabling accurate thiol identification.

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Area of Science:

Analytical Chemistry
Biochemistry

Background:

Thiols are vital in physiological processes.
Discriminating between different thiols is challenging but important.

Purpose of the Study:

To develop a facile colorimetric sensor array for enhanced thiol discrimination.
To leverage urease-metal ion interactions for sensitive thiol detection.

Main Methods:

Fabrication of a sensor array using urease-metal ion pairs.
Exploiting differential thiol affinities for metal ions and urease activation.
Utilizing linear discriminant analysis (LDA) for pattern recognition.

Main Results:

The sensor array successfully distinguished cysteine (Cys), glutathione (GSH), and four other thiols at low concentrations (1.0 μM).
Generated distinct colorimetric response patterns characteristic of each thiol.
Achieved high accuracy (96.67%) in identifying 30 unknown thiol samples.

Conclusions:

The proposed sensor array offers a sensitive and facile method for thiol discrimination.
Urease and its metal ion inhibitors provide a cost-effective platform for developing enzyme-based sensing systems.