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Updated: Mar 16, 2026
Detection and Genogrouping of Noroviruses from Children's Stools By Taqman One-step RT-PCR
Published on: July 22, 2012
[Genotypes of adenoviruses in infants and young children with diarrhea]
Qiu-Li Zhang1, Hong-Bo Wang, Yan-Ling Wang
1Department of Pediatrics, First Hospital of Shizuishan Affiliated to Ningxia Medical University, Shizuishan, Ningxia 753200, China. zql128109@163.com.
Insights
Adenoviruses (AdV) cause diarrhea in young children, particularly those aged 2-3 years. Enteric adenovirus 41 (AdV41) is the most common strain identified in these cases.
Area of Science:
- Virology
- Pediatric Infectious Diseases
- Gastroenterology
Background:
- Adenoviruses (AdV) are a common cause of viral infections in children.
- Diarrhea is a significant health concern in infants and young children globally.
- Understanding AdV prevalence and genotypes is crucial for diagnosis and management.
Purpose of the Study:
- To determine the prevalence of adenoviruses (AdV) in young children presenting with diarrhea.
- To identify the specific genotypes of AdV circulating in this pediatric population.
Main Methods:
- Stool samples were collected from 380 children under 3 years old with diarrhea.
- Genomic DNA was extracted, and polymerase chain reaction (PCR) was used for AdV detection.
- Positive samples underwent clone sequencing and genotyping to identify AdV types.
Main Results:
- Adenovirus was detected in 6.3% (24/380) of the children studied.
- The majority of AdV-positive cases were observed in children aged 2-3 years.
- Enteric AdV41 was the predominant genotype (4.2%), followed by non-enteric AdVs (2.1%) including AdV1, AdV2, AdV7, AdV12, and AdV31.
Conclusions:
- Adenovirus infection, particularly AdV41, is a significant cause of diarrhea in children aged 2-3 years.
- Genotyping reveals a mix of enteric and non-enteric AdV strains contributing to pediatric diarrhea.
- Findings highlight the importance of AdV surveillance in pediatric gastroenteritis.
Objective:
To investigate the prevalence of adenoviruses (AdV) and their genotypes in infants and young children with diarrhea.
Methods:
A total of 380 children with diarrhea aged less than 3 years were enrolled. The genomic DNA was extracted from stool and PCR was used to detect AdV. Clone sequencing and genotyping were performed for DNA in AdV-positive specimens.
Results:
AdV was detected in 24 out of 380 specimens, and the detection rate was 6.3% (24/380). A majority of children with positive AdV were aged 2-3 years. The viral sequence analysis of positive specimens showed that the detection rates of enteric AdV41 and non-enteric AdV were 4.2% (16/380) and 2.1% (8/380), respectively, and among the children with non-enteric AdV, there were 2 with AdV1, 2 with AdV2, 1 with AdV7, 2 with AdV12, and 1 with AdV31.
Conclusions:
Diarrhea caused by AdV is commonly seen in children aged 2-3 years, and AdV41 is the major predominant strain.
