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Sequence selective binding of ditrisarubicin B to DNA: comparison with daunomycin
1Dept of Physiology & Pharmacology, University of Southampton, England.
FEBS Letters
|July 3, 1989
Abstract:
DNase I footprinting has been used to examine the sequence selective binding of ditrisarubicin B, a novel anthracycline antibiotic, to DNA. At 37 degrees C no footprinting pattern is observed, the drug protects all sites from enzymic cleavage with equal efficiency. At 4 degrees C a footprinting pattern is induced with low drug concentrations which is different from that produced by daunomycin. The best binding sites contain the dinucleotide step GpT (ApC) and are located in regions of alternating purines and pyrimidines.