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[Effect of Icaritin on K562 Cell Proliferation and Intracellular Reactive Oxygen Species]
Ting Ma1, Zi-Jian Li1, Li-Na Wang1
1Department of Hematology,The First Hospital of Lanzhou University, Lanzhou 730000, Gansu Province, China.
Objective:
To investigate the effect of icaritin (ICT) on proliferation of K562 cells and reactive oxygen species (ROS) in patients with chronic myeloid leukemia (CML).
Methods:
MTT assay was used to detect the effect of ICT on the proliferation of K562 cells. Flow cytometry was used to detect the apoptosis of K562 cells and intracellular ROS level. The expression of PARP protein was detected by Western blot.
Results:
ICT obviously inhibited the proliferation of K562 cells and induced their apoptosis, the expression of PARP protein was enhanced, and the intracellular ROS increased significantly.
Conclusion:
The ICT showes the inhibitory effects on proliferation and apoptosis-inducing effects on K562 cells, and thier mechanism relats with the increase of reactive oxygen species in the cells.
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