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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Database-guided Flow-cytometry for Evaluation of Bone Marrow Myeloid Cell Maturation

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Malignant Nonhematological Effusion Characterization by Flow Cytometry.

¹Department of Pathology, Oslo University Hospital, Norwegian Radium Hospital, and Institute of Clinical Medicine, Faculty of Medicine, University of Oslo, Oslo, Norway.

Acta Cytologica

|August 18, 2016

Summary

This summary is machine-generated.

Flow cytometry (FC) offers quantitative analysis for diagnosing malignant effusions by differentiating cell types and detecting cancer markers. This method shows potential in cancer diagnosis, prediction, and prognosis, though not yet routine for treatment monitoring.

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Area of Science:

Oncology
Immunology
Cell Biology

Background:

Flow cytometry (FC) is underutilized in diagnosing malignant effusions, except for hematological malignancies.
FC provides quantitative analysis differentiating epithelial, mesothelial, and leukocyte cells using specific antibodies.

Purpose of the Study:

To review the current applications of FC in the diagnostic and research settings for serous effusions.
To highlight FC's potential in cancer diagnosis, prediction, and prognosis using clinical specimens.

Main Methods:

Review of current literature on flow cytometry applications in serous effusions.
Analysis of FC's ability to identify cell types and cancer-associated molecules.

Main Results:

FC effectively distinguishes between epithelial cells, mesothelial cells, and leukocytes.
FC enables quantitative detection of cancer markers, proliferation, and apoptosis.
Potential for monitoring treatment response in metastatic disease.

Conclusions:

Flow cytometry is a valuable ancillary tool for diagnosing malignant effusions.
FC's quantitative capabilities support diagnosis, prediction, and prognosis in cancer patients.
Further research may integrate FC into routine clinical practice for effusion analysis.