Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

13.6K
Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
13.6K
Fluorescence and Phosphorescence: Instrumentation01:25

Fluorescence and Phosphorescence: Instrumentation

1.8K
Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.
1.8K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

21.7K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
21.7K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

14.7K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
14.7K
Photoluminescence: Fluorescence and Phosphorescence01:23

Photoluminescence: Fluorescence and Phosphorescence

4.4K
Photoluminescence is a process where a molecule absorbs light energy and re-emits it in the form of light. This phenomenon occurs when a substance absorbs photons, promoting its electrons to higher energy level excited states, followed by a relaxation process in which the electrons return to their original ground state energy levels and emit light. Photoluminescence is widely observed in various materials, including semiconductors, and organic and inorganic compounds.
A pair of electrons in a...
4.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Lipoprotein subfractions in cardiovascular disease risk prediction and therapeutic targeting: Current landscape and future directions.

Journal of translational internal medicine·2026
Same author

AQP1 Suppresses Clear Cell Renal Cell Carcinoma via Epigenetic Silencing and TNF-Mediated Apoptosis.

International journal of molecular sciences·2026
Same author

The Prognostic and Mutational Characteristics of Multiple Early-stage Lung Cancers Manifesting as Subsolid Nodules.

Current medical imaging·2026
Same author

Fractographic Analysis and Fatigue Crack Propagation Behavior of TC4-F Alloy with a Duplex Microstructure.

Materials (Basel, Switzerland)·2026
Same author

WD repeat-containing protein 41 (WDR41) is involved in fertilization and interacts with IZUMO1 and JUNO.

Theriogenology·2026
Same author

Mapping neutralizing epitopes and developing protective chimeric antibodies against porcine epidemic diarrhea virus infection.

International journal of biological macromolecules·2026
Same journal

Method of spatial scanning of modulated laser radiation for outline imaging of interphalangeal joints.

Journal of biomedical optics·2026
Same journal

Multimodal optical imaging for the assessment of the teratogenic effects of ethanol on zebrafish development.

Journal of biomedical optics·2026
Same journal

Fluorescence properties of collagen types I-V: a comprehensive study of spectral and lifetime characteristics.

Journal of biomedical optics·2026
Same journal

Spectral dependence of lipofuscin fluorescence lifetimes revealed by FLIM with a superconducting nanowire single-photon detector.

Journal of biomedical optics·2026
Same journal

Building the future of biophotonics through experiential education and seasonal schools.

Journal of biomedical optics·2026
Same journal

Time-of-flight fluorescence depth mapping using a spatiotemporal deep learning model.

Journal of biomedical optics·2026
See all related articles

Related Experiment Video

Updated: Mar 16, 2026

A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins
16:10

A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins

Published on: March 22, 2012

24.5K

Fluorescence Talbot microscope using incoherent source.

Yangyang Sun, Shuo Pang

    Journal of Biomedical Optics
    |August 18, 2016
    PubMed
    Summary
    This summary is machine-generated.

    This study introduces a cost-effective Fluorescence Talbot microscope using a light-emitting diode for broader applications. The new system achieves high illumination intensity and comparable image quality to traditional microscopes.

    More Related Videos

    Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
    12:51

    Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

    Published on: December 9, 2013

    9.4K
    Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy
    15:04

    Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy

    Published on: May 18, 2011

    13.6K

    Related Experiment Videos

    Last Updated: Mar 16, 2026

    A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins
    16:10

    A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins

    Published on: March 22, 2012

    24.5K
    Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
    12:51

    Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

    Published on: December 9, 2013

    9.4K
    Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy
    15:04

    Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy

    Published on: May 18, 2011

    13.6K

    Area of Science:

    • Microscopy
    • Optical Imaging
    • Biotechnology

    Background:

    • Talbot fluorescence microscopy offers scalable field-of-view (FOV) imaging with microscopic resolution.
    • Existing systems require coherent illumination, limiting use in low-resource settings.
    • A cost-effective alternative is needed for wider accessibility.

    Purpose of the Study:

    • To demonstrate a cost-effective Fluorescence Talbot microscope setup.
    • To overcome the limitations of coherent illumination in previous systems.
    • To enable broad applications in various settings.

    Main Methods:

    • Utilized a light-emitting diode (LED) with a specific wavelength (16 nm FWHM) and small area (4 mm²).
    • Implemented a Talbot fluorescence system without relying on spatially filtered, coherent illumination.
    • Achieved significantly higher illumination intensity compared to single pinhole filtering.

    Main Results:

    • Demonstrated a 357-fold increase in illumination intensity compared to single pinhole methods.
    • Reconstructed image quality was comparable to a 10× microscope objective.
    • Successfully imaged various samples, including fluorescent beads, HeLa cells, and mouse kidney tissue.

    Conclusions:

    • The developed LED-based Fluorescence Talbot microscope is a viable, cost-effective imaging solution.
    • This system expands the potential applications of Talbot fluorescence microscopy, especially in resource-limited environments.
    • The technology maintains high resolution and image quality for diverse biological samples.