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Cryo-electron Microscopy

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...

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Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging

Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging

Published on: July 12, 2022

Biological cryo-electron microscopy in China.

Hong-Wei Wang¹, Jianlin Lei¹, Yigong Shi¹

¹Beijing Advanced Innovation Center for Structural Biology, School of Life Sciences, Tsinghua University, Beijing, 100084, China.

Protein Science : a Publication of the Protein Society

|August 19, 2016

Summary

Biological cryo-electron microscopy (cryo-EM) is rapidly advancing in China, driven by new facilities. This review covers its history, current impact, and future prospects in structural biology.

Keywords:

China EM methodology Tsinghua University cryo-EM electron microscopy history transmission electron microscope

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Area of Science:

Structural Biology
Biophysics
Biochemistry

Background:

Biological cryo-electron microscopy (cryo-EM) is crucial for determining molecular structures.
China's National Protein Science Facility expansion has accelerated cryo-EM development.
Significant progress in cryo-EM has been observed in China.

Purpose of the Study:

To review the historical development of biological cryo-EM in China.
To describe the current status and impact of cryo-EM in Chinese biological research.
To provide an outlook on the future of cryo-EM in China.

Main Methods:

Literature review of biological cryo-EM in China.
Analysis of current cryo-EM infrastructure and applications.
Discussion of future trends and potential advancements.

Main Results:

Biological cryo-EM has a growing importance in China.
The establishment of advanced facilities has spurred rapid development.
Cryo-EM is significantly impacting various biological research fields.

Conclusions:

China is emerging as a key player in biological cryo-EM.
Continued investment and research will further enhance cryo-EM capabilities.
The future outlook for cryo-EM in China is promising for structural biology.