Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Renewal of Intestinal Stem Cells

Renewal of Intestinal Stem Cells

The intestinal epithelial lining rapidly renews every 4 to 5 days. The renewal is facilitated by intestinal stem cells (ISCs) located at the base of the crypt– a gland located at the bottom of each villus. ISCs divide asymmetrically to form new stem cells and progenitor daughter cells. The daughter cells are called transit-amplifying (TA) cells which move upwards along the crypt and either differentiate into absorptive cells– the enterocytes or secretory cells– including the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Loeffler's Endomyocarditis: Clinical characteristics and outcomes from a tertiary care centre registry.

International journal of cardiology·2026

Same author

Patient-derived tissue slice cultures from endoscopic biopsies as a translational ex vivo model for inflammatory bowel diseases.

Clinical and experimental medicine·2026

Same author

Development of phenotype algorithms for the detection of adverse events in electronic health record data: a multicentre study.

BMJ open·2026

Same author

A distributed analysis approach for pharmacovigilance data from electronic medical records in German university hospitals: the POLAR_MI ETL Pipeline.

BMC medical informatics and decision making·2026

Same author

Secondary Data for Clinical Pharmacists' Decision Support: Evaluating 'Triple Whammy' Interactions Within INTERPOLAR.

Studies in health technology and informatics·2026

Same author

Detecting Contraindications in Routinely Collected Healthcare Data to Emulate Decision Support for Medication Reviews Within the INTERPOLAR Study.

Studies in health technology and informatics·2026

Search research articles

Related Experiment Video

Updated: Mar 16, 2026

Author Spotlight: Developing Immunocompetent Organ-on-Chip Models for Infectious Disease Research

Author Spotlight: Developing Immunocompetent Organ-on-Chip Models for Infectious Disease Research

Published on: May 24, 2024

Stem cell competition in the gut: insights from multi-scale computational modelling.

Torsten Thalheim¹, Peter Buske¹, Jens Przybilla¹

¹Interdisciplinary Center for Bioinformatics, Leipzig University, Haertelstr. 16-18, 04107 Leipzig, Germany.

Journal of the Royal Society, Interface

|August 19, 2016

Summary

Three-dimensional computational tissue models reveal how cellular dynamics and signaling pathways influence intestinal tissue. These models predict that Notch signaling suppresses Wnt-driven secretory cell development, increasing tumor-causing mutation fixation.

Keywords:

Paneth cell specification Wnt and Notch signalling clonal competition mouse small intestine three-dimensional computational model

More Related Videos

Author Spotlight: Enhancing Understanding and Treatment Strategies with the NEC-on-a-Chip Model

Author Spotlight: Enhancing Understanding and Treatment Strategies with the NEC-on-a-Chip Model

Published on: July 28, 2023

A Gut-on-a-Chip Model to Study the Gut Microbiome-Nervous System Axis

A Gut-on-a-Chip Model to Study the Gut Microbiome-Nervous System Axis

Published on: July 28, 2023

Related Experiment Videos

Last Updated: Mar 16, 2026

Author Spotlight: Developing Immunocompetent Organ-on-Chip Models for Infectious Disease Research

Author Spotlight: Developing Immunocompetent Organ-on-Chip Models for Infectious Disease Research

Published on: May 24, 2024

Author Spotlight: Enhancing Understanding and Treatment Strategies with the NEC-on-a-Chip Model

Author Spotlight: Enhancing Understanding and Treatment Strategies with the NEC-on-a-Chip Model

Published on: July 28, 2023

A Gut-on-a-Chip Model to Study the Gut Microbiome-Nervous System Axis

A Gut-on-a-Chip Model to Study the Gut Microbiome-Nervous System Axis

Published on: July 28, 2023

Area of Science:

Computational biology
Systems biology
Tissue engineering

Background:

Three-dimensional (3D) computational tissue models offer detailed insights into molecular, cellular, and tissue-level dynamics.
These models facilitate hypothesis generation regarding cellular interactions and signaling pathway synergies.

Purpose of the Study:

To simulate a 3D single-cell-based model of mouse small intestinal crypts.
To analyze the impact of lineage specification, distribution, and cellular lifespan on clonal competition.
To investigate the effects of Notch and Wnt pathway activation on mutation fixation within the tissue.

Main Methods:

Development and simulation of a 3D single-cell-based computational model.
Analysis of lineage specification, cellular distribution, and lifespan effects on clonal dynamics.
Modeling of Notch and Wnt signaling pathway activation and their influence on mutation fixation.

Main Results:

Simulations revealed the impact of lineage specification, distribution, and lifespan on clonal competition in intestinal crypts.
Notch and Wnt pathway activation were studied for their effects on mutation fixation.
A prediction was made regarding the synergistic effects of Notch and Wnt signaling in intestinal tumorigenesis.

Conclusions:

3D computational tissue models can mechanistically explain long-term tissue dynamics during homeostasis and transformation.
The study predicts that Notch signaling suppresses Wnt-dependent secretory lineage specification, enhancing Wnt-activating mutation fixation.
This mechanism explains observed synergistic effects of Notch and Wnt signaling in intestinal tumorigenesis.